PMID:10722597

Citation	Foynes, S, Dorrell, N, Ward, SJ, Stabler, RA, McColm, AA, Rycroft, AN and Wren, BW (2000) Helicobacter pylori possesses two CheY response regulators and a histidine kinase sensor, CheA, which are essential for chemotaxis and colonization of the gastric mucosa. Infect. Immun. 68:2016-23
Abstract	Infection of the mucous layer of the human stomach by Helicobacter pylori requires the bacterium to be motile and presumably chemotactic. Previous studies have shown that fully functional flagella are essential for motility and colonization, but the role of chemotaxis remains unclear. The two-component regulatory system CheA/CheY has been shown to play a major role in chemotaxis in other enteric bacteria. Scrutiny of the 26695 genome sequence suggests that H. pylori has two CheY response regulators: one a separate protein (CheY1) and the other (CheY2) fused to the histidine kinase sensor CheA. Defined deletion mutations were introduced into cheY1, cheY2, and cheA in H. pylori strains N6 and SS1. Video tracking revealed that the wild-type H. pylori strain moves in short runs with frequent direction changes, in contrast to movement of cheY2, cheAY2, and cheAY2 cheY1 mutants, whose motion was more linear. The cheY1 mutant demonstrated a different motility phenotype of rapid tumbling. All mutants had impaired swarming and greatly reduced chemotactic responses to hog gastric mucin. Neither cheY1 nor cheAY2 mutants were able to colonize mice, but they generated a significant antibody response, suggesting that despite impaired chemotaxis, these mutants were able to survive in the stomach long enough to induce an immune response before being removed by gastric flow. Additionally, we demonstrated that cheY1 failed to colonize gnotobiotic piglets. This study demonstrates the importance of the roles of cheY1, cheY2, and cheA in motility and virulence of H. pylori.
Links	PubMed PMC97381
Keywords	Animals; Bacterial Proteins; Blood/microbiology; Chemotaxis; Enzyme-Linked Immunosorbent Assay; Escherichia coli/metabolism; Female; Flagella/genetics; Gastric Mucosa/microbiology; Helicobacter pylori/enzymology; Helicobacter pylori/genetics; Helicobacter pylori/pathogenicity; Membrane Proteins/genetics; Membrane Proteins/physiology; Mice; Movement; Mutagenesis, Site-Directed; Swine; Virulence
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HELPX:I0ZC23	involved_in	GO:0052116: chemotaxis in host environment	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
HELPX:I0ZC23		GO:0052116: chemotaxis in host environment	ECO:0000315:		P		Table 3 shows a significant loss of chemotaxis function in the CheY1 and CheY2 mutants	complete CACAO 9857
HELPX:I0ZHF1	involved_in	GO:0052116: chemotaxis in host environment	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
HELPX:I0ZHF1		GO:0052116: chemotaxis in host environment	ECO:0000315:		P		Table 3 shows significantly reduced chemotaxis in CheA mutants.	complete CACAO 10013

References

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