PMID:10714992

Citation	Takamatsu, H, Kodama, T, Imamura, A, Asai, K, Kobayashi, K, Nakayama, T, Ogasawara, N and Watabe, K (2000) The Bacillus subtilis yabG gene is transcribed by SigK RNA polymerase during sporulation, and yabG mutant spores have altered coat protein composition. J. Bacteriol. 182:1883-8
Abstract	The expression of six novel genes located in the region from abrB to spoVC of the Bacillus subtilis chromosome was analyzed, and one of the genes, yabG, had a predicted promoter sequence conserved among SigK-dependent genes. Northern blot analysis revealed that yabG mRNA was first detected from 4 h after the cessation of logarithmic growth (T(4)) in wild-type cells and in a gerE36 (GerE(-)) mutant but not in spoIIAC (SigF(-)), spoIIGAB (SigE(-)), spoIIIG (SigG(-)), and spoIVCB (SigK(-)) mutants. The transcription start point was determined by primer extension analysis; the -10 and -35 regions are very similar to the consensus sequences recognized by SigK-containing RNA polymerase. Inactivation of the yabG gene by insertion of an erythromycin resistance gene did not affect vegetative growth or spore resistance to heat, chloroform, and lysozyme. The germination of yabG spores in L-alanine and in a mixture of L-asparagine, D-glucose, D-fructose, and potassium chloride was also the same as that of wild-type spores. On the other hand, the protein preparation from yabG spores included 15-, 18-, 21-, 23-, 31-, 45-, and 55-kDa polypeptides which were low in or not extracted from wild-type spores under the same conditions. We determined their N-terminal amino acid sequence and found that these polypeptides were CotT, YeeK, YxeE, CotF, YrbA (31 and 45 kDa), and SpoIVA, respectively. The fluorescence of YabG-green fluorescent protein fusion produced in sporulating cells was detectable in the forespores but not in the mother cell compartment under fluorescence microscopy. These results indicate that yabG encodes a sporulation-specific protein which is involved in coat protein composition in B. subtilis.
Links	PubMed PMC101870
Keywords	Alanine/metabolism; Amino Acid Sequence; Bacillus subtilis/enzymology; Bacillus subtilis/genetics; Bacillus subtilis/physiology; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Chloroform/metabolism; Consensus Sequence/genetics; DNA-Directed RNA Polymerases/metabolism; Gene Expression Regulation, Bacterial/genetics; Genes, Bacterial; Hot Temperature; Molecular Sequence Data; Molecular Weight; Muramidase/metabolism; Mutation/genetics; Peptides/analysis; Peptides/chemistry; Promoter Regions, Genetic/genetics; RNA, Messenger/analysis; RNA, Messenger/genetics; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Sigma Factor; Spores, Bacterial/chemistry; Spores, Bacterial/genetics; Spores, Bacterial/metabolism; Spores, Bacterial/physiology; Transcription Factors/physiology; Transcription, Genetic/genetics
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BACSU:YABG		GO:0043593: endospore coat	ECO:0000315:		C		Figure 5. shows SDS-PAGE results that show a significant difference in the protein profile of yabG mutant spores and wild-type spores. This led researchers to infer that yabG is sporulation specific and involved in coat protein composition.	complete CACAO 6141
BACSU:YABG	part_of	GO:0043593: endospore coat	ECO:0000315: mutant phenotype evidence used in manual assertion		C		Seeded From UniProt	complete

References

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