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PMID:10684247
| Citation |
Rout, MP, Aitchison, JD, Suprapto, A, Hjertaas, K, Zhao, Y and Chait, BT (2000) The yeast nuclear pore complex: composition, architecture, and transport mechanism. J. Cell Biol. 148:635-51 |
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| Abstract |
An understanding of how the nuclear pore complex (NPC) mediates nucleocytoplasmic exchange requires a comprehensive inventory of the molecular components of the NPC and a knowledge of how each component contributes to the overall structure of this large molecular translocation machine. Therefore, we have taken a comprehensive approach to classify all components of the yeast NPC (nucleoporins). This involved identifying all the proteins present in a highly enriched NPC fraction, determining which of these proteins were nucleoporins, and localizing each nucleoporin within the NPC. Using these data, we present a map of the molecular architecture of the yeast NPC and provide evidence for a Brownian affinity gating mechanism for nucleocytoplasmic transport. |
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| Keywords |
Biological Transport; Carrier Proteins/analysis; Carrier Proteins/chemistry; Carrier Proteins/genetics; Chromatography, High Pressure Liquid; Fluorescent Antibody Technique; Fungal Proteins/analysis; Fungal Proteins/chemistry; Fungal Proteins/genetics; Membrane Proteins/analysis; Membrane Proteins/chemistry; Membrane Proteins/genetics; Microscopy, Immunoelectron; Models, Biological; Molecular Weight; Nuclear Envelope/chemistry; Nuclear Envelope/genetics; Nuclear Envelope/metabolism; Nuclear Envelope/ultrastructure; Nuclear Proteins/analysis; Nuclear Proteins/chemistry; Nuclear Proteins/genetics; Recombinant Fusion Proteins/analysis; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/genetics; Saccharomyces cerevisiae/chemistry; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae/ultrastructure; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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