PMID:10677040

Citation	Nurrish, S, Ségalat, L and Kaplan, JM (1999) Serotonin inhibition of synaptic transmission: Galpha(0) decreases the abundance of UNC-13 at release sites. Neuron 24:231-42
Abstract	We show that serotonin inhibits synaptic transmission at C. elegans neuromuscular junctions, and we describe a signaling pathway that mediates this effect. Release of acetylcholine from motor neurons was assayed by measuring the sensitivity of intact animals to the acetylcholinesterase inhibitor aldicarb. By this assay, exogenous serotonin inhibited acetylcholine release, whereas serotonin antagonists stimulated release. The effects of serotonin on synaptic transmission were mediated by GOA-1 (a Galpha0 subunit) and DGK-1 (a diacylglycerol [DAG] kinase), both of which act in the ventral cord motor neurons. Mutants lacking goa-1 G(alpha)0 accumulated abnormally high levels of the DAG-binding protein UNC-13 at motor neuron nerve terminals, suggesting that serotonin inhibits synaptic transmission by decreasing the abundance of UNC-13 at release sites.
Links	PubMed
Keywords	Acetylcholine/secretion; Aldicarb/pharmacology; Amino Acid Sequence; Animals; Caenorhabditis elegans; Caenorhabditis elegans Proteins; Cell Line; Cholinesterase Inhibitors/pharmacology; Diacylglycerol Kinase/chemistry; Diacylglycerol Kinase/genetics; Diacylglycerol Kinase/metabolism; GTP-Binding Protein alpha Subunits, Gi-Go; Helminth Proteins/metabolism; Heterotrimeric GTP-Binding Proteins/chemistry; Heterotrimeric GTP-Binding Proteins/genetics; Heterotrimeric GTP-Binding Proteins/physiology; Molecular Sequence Data; Motor Neurons/drug effects; Motor Neurons/metabolism; Mutation; Serotonin/pharmacology; Signal Transduction; Synaptic Transmission/drug effects
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