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PMID:10660671
| Citation |
Chase, D, Serafinas, C, Ashcroft, N, Kosinski, M, Longo, D, Ferris, DK and Golden, A (2000) The polo-like kinase PLK-1 is required for nuclear envelope breakdown and the completion of meiosis in Caenorhabditis elegans. Genesis 26:26-41 |
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| Abstract |
The Polo-like kinases are key regulatory molecules required during the cell cycle for the successful completion of mitosis. We have cloned a C. elegans homolog of the Drosophila melanogaster polo gene (designated plk-1 for C. elegans polo-like kinase-1) and present the subcellular localization of the PLK-1 protein during the meiotic and mitotic cell cycles in C. elegans oocytes and embryos, respectively. Disruption of PLK-1 expression by RNA-mediated interference (RNAi) disrupts normal oocyte and embryonic development. Inspection of oocytes revealed a defect in nuclear envelope breakdown (NEBD) before ovulation. This defect in NEBD was also observed in oocytes that were depleted of the cyclin-dependent kinase NCC-1 (C. elegans homolog of Cdc2). The plk-1 RNAi oocytes were fertilized; however the resulting embryos were unable to separate their meiotic chromosomes or form and extrude polar bodies. These defects led to embryonic arrest as single cells. genesis 26:26-41, 2000. Published 2000 Wiley-Liss, Inc. |
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| Keywords |
Amino Acid Sequence; Animals; Base Sequence; Caenorhabditis elegans/embryology; Caenorhabditis elegans/enzymology; Caenorhabditis elegans Proteins; Cell Cycle Proteins; DNA, Complementary/genetics; Embryo, Nonmammalian/drug effects; Embryo, Nonmammalian/enzymology; Embryo, Nonmammalian/ultrastructure; Enzyme Induction/drug effects; Gene Expression Regulation, Developmental/drug effects; Genes, Helminth; Helminth Proteins/genetics; Helminth Proteins/physiology; Meiosis/physiology; Mitosis/physiology; Molecular Sequence Data; Nuclear Envelope/physiology; Oocytes/enzymology; Oocytes/ultrastructure; Protein Kinases/deficiency; Protein Kinases/genetics; Protein Kinases/physiology; Protein-Serine-Threonine Kinases; Proto-Oncogene Proteins; RNA, Double-Stranded/pharmacology; Sequence Alignment; Sequence Homology, Amino Acid |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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