PMID:10644706

Citation	Ritz, D, Patel, H, Doan, B, Zheng, M, Aslund, F, Storz, G and Beckwith, J (2000) Thioredoxin 2 is involved in the oxidative stress response in Escherichia coli. J. Biol. Chem. 275:2505-12
Abstract	Two genes encoding thioredoxin are found on the Escherichia coli genome. Both of them are capable of reducing protein disulfide bonds in vivo and in vitro. The catalytic site contains a Cys-X(1)-X(2)-Cys motif in a so-called thioredoxin fold. Thioredoxin 2 has two additional pairs of cysteines in a non-conserved N-terminal domain. This domain does not appear to be important for the function of thioredoxin 2 in donating electrons to ribonucleotide reductase, 3'-phosphoadenylsulfate-reductase, or the periplasmic disulfide isomerase DsbC. Our results suggests that the two thioredoxins are equivalent for most of the in vivo functions that were tested. On the other hand, transcriptional regulation is different. The expression of trxC is regulated by the transcriptional activator OxyR in response to oxidative stress. Oxidized OxyR binds directly to the trxC promoter and induces its expression in response to elevated hydrogen peroxide levels or the disruption of one or several of the cytoplasmic redox pathways. Mutants lacking thioredoxins 1 and 2 are more resistant to high levels of hydrogen peroxide, whereas they are more sensitive to diamide, a disulfide bond-inducing agent.
Links	PubMed
Keywords	Base Sequence; DNA, Bacterial; DNA-Binding Proteins; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Escherichia coli Proteins; Molecular Sequence Data; Mutagenesis, Site-Directed; Oxidative Stress; Protein Binding; Protein Isoforms/genetics; Protein Isoforms/metabolism; Repressor Proteins/metabolism; Thioredoxins/genetics; Thioredoxins/metabolism; Trans-Activators/metabolism; Transcription Factors/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ECOLI:THIO2		GO:0047134: protein-disulfide reductase activity	ECO:0000315:		F		Fig 1	complete CACAO 7967
ECOLI:THIO2	enables	GO:0047134: protein-disulfide reductase activity	ECO:0000315: mutant phenotype evidence used in manual assertion		F		Seeded From UniProt	complete

References

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