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PMID:10636868
| Citation |
Choi, H, Hong, J, Ha, J, Kang, J and Kim, SY (2000) ABFs, a family of ABA-responsive element binding factors. J. Biol. Chem. 275:1723-30 |
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| Abstract |
Abscisic acid (ABA) plays an important role in environmental stress responses of higher plants during vegetative growth. One of the ABA-mediated responses is the induced expression of a large number of genes, which is mediated by cis-regulatory elements known as abscisic acid-responsive elements (ABREs). Although a number of ABRE binding transcription factors have been known, they are not specifically from vegetative tissues under induced conditions. Considering the tissue specificity of ABA signaling pathways, factors mediating ABA-dependent stress responses during vegetative growth phase may thus have been unidentified so far. Here, we report a family of ABRE binding factors isolated from young Arabidopsis plants under stress conditions. The factors, isolated by a yeast one-hybrid system using a prototypical ABRE and named as ABFs (ABRE binding factors) belong to a distinct subfamily of bZIP proteins. Binding site selection assay performed with one ABF showed that its preferred binding site is the strong ABRE, CACGTGGC. ABFs can transactivate an ABRE-containing reporter gene in yeast. Expression of ABFs is induced by ABA and various stress treatments, whereas their induction patterns are different from one another. Thus, a new family of ABRE binding factors indeed exists that have the potential to activate a large number of ABA/stress-responsive genes in Arabidopsis. |
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| Keywords |
Abscisic Acid/metabolism; Amino Acid Sequence; Arabidopsis/metabolism; Base Sequence; Binding Sites; Gene Expression Regulation, Plant; Gene Library; Molecular Sequence Data; Phylogeny; Plant Proteins/metabolism; Protein Binding; Recombinant Proteins/metabolism; Response Elements; Reverse Transcriptase Polymerase Chain Reaction; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Substrate Specificity; Time Factors; Transcriptional Activation; Two-Hybrid System Techniques |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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