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PMID:10625546
| Citation |
Inoue, T and Thomas, JH (2000) Targets of TGF-beta signaling in Caenorhabditis elegans dauer formation. Dev. Biol. 217:192-204 |
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| Abstract |
Caenorhabditis elegans dauer formation is controlled by multiple environmental factors. The chemosensory neuron ASI regulates dauer formation by secretion of DAF-7/TGF-beta, but the molecular targets of the DAF-7 ligand are incompletely defined and the cellular targets are unknown. We genetically characterized and cloned a putative transducer of DAF-7 signaling called daf-14 and found that it encodes a Smad protein. DAF-14 Smad has a highly unusual structure completely lacking the N-terminal domain found in all other Smad proteins known to date. daf-14 genetically interacts with daf-8, which encodes another Smad, and the interaction suggests partial functional redundancy between these two Smad proteins. We also studied the cellular targets of DAF-7 signaling by studying the sites of action of daf-14 and daf-4, the putative receptor for DAF-7. daf-14::gfp is expressed in multiple tissues that are remodeled during dauer formation. However, analysis of mosaics generated by free duplication loss and tissue-specific expression constructs indicate cell-nonautonomous function of daf-4, arguing against direct DAF-7 signaling to tissues throughout the animal. Instead, these experiments suggest the nervous system as a target of DAF-7 signaling and that the nervous system in turn regulates dauer formation by other tissues. |
| Links |
PubMed Online version:10.1006/dbio.1999.9545 |
| Keywords |
Alleles; Amino Acid Sequence; Animals; Animals, Genetically Modified; Caenorhabditis elegans/genetics; Caenorhabditis elegans/metabolism; Caenorhabditis elegans Proteins; Cloning, Molecular; DNA-Binding Proteins/chemistry; DNA-Binding Proteins/genetics; Green Fluorescent Proteins; Luminescent Proteins/genetics; Molecular Sequence Data; Mosaicism; Promoter Regions, Genetic; Recombinant Fusion Proteins/genetics; Signal Transduction; Smad Proteins; Trans-Activators/chemistry; Trans-Activators/genetics; Transforming Growth Factor beta/metabolism |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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