PMID:10587644

Citation	Feng, H, Zhong, W, Punkosdy, G, Gu, S, Zhou, L, Seabolt, EK and Kipreos, ET (1999) CUL-2 is required for the G1-to-S-phase transition and mitotic chromosome condensation in Caenorhabditis elegans. Nat. Cell Biol. 1:486-92
Abstract	The human cullin protein CUL-2 functions in a ubiquitin-ligase complex with the von Hippel-Lindau (VHL) tumour suppressor protein. Here we show that, in Caenorhabditis elegans, cul-2 is expressed in proliferating cells and is required at two distinct points in the cell cycle, the G1-to-S-phase transition and mitosis. cul-2 mutant germ cells undergo a G1-phase arrest that correlates with accumulation of CKI-1, a member of the CIP/KIP family of cyclin-dependent-kinase inhibitors. In cul-2 mutant embryos, mitotic chromosomes are unable to condense, leading to unequal DNA segregation, chromosome bridging and the formation of multiple nuclei.
Links	PubMed Online version:10.1038/70272
Keywords	Amino Acid Sequence; Animals; Caenorhabditis elegans/cytology; Caenorhabditis elegans/embryology; Caenorhabditis elegans/genetics; Caenorhabditis elegans/metabolism; Caenorhabditis elegans Proteins; Cell Cycle Proteins/chemistry; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Cell Lineage; Cell Nucleus/genetics; Chromosomes/chemistry; Chromosomes/genetics; Chromosomes/metabolism; Cloning, Molecular; Cullin Proteins; Cyclin-Dependent Kinase Inhibitor Proteins; Fluorescent Antibody Technique; G1 Phase; Gene Expression Regulation, Developmental; Germ Cells/cytology; Germ Cells/metabolism; In Situ Hybridization; Mitosis; Molecular Conformation; Molecular Sequence Data; Mutation/genetics; Nondisjunction, Genetic; Phenotype; RNA, Messenger/analysis; RNA, Messenger/genetics; S Phase
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