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PMID:1057168
| Citation |
Clarke, M, Schatten, G, Mazia, D and Spudich, JA (1975) Visualization of actin fibers associated with the cell membrane in amoebae of Dictyostelium discoideum. Proc. Natl. Acad. Sci. U.S.A. 72:1758-62 |
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| Abstract |
Amoebae of Dictyostelium discoideum were attached to a surface coated with polylysine, and the upper portion of the cells was sheared off with a stream of buffer. Scanning and transmission electron microscopy showed that the cytoplasmic surface of the exposed membrane was covered with fibers consisting of actin-containing filaments. The actin was identified by its solubility properties and its ability to interact with muscle myosin. |
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| Keywords |
Actins/analysis; Amoeba/analysis; Amoeba/ultrastructure; Binding Sites; Cell Membrane/analysis; Cell Membrane/ultrastructure; Dictyostelium/analysis; Dictyostelium/ultrastructure; Klebsiella; Microscopy, Electron; Microscopy, Electron, Scanning; Myxomycetes/analysis; Polylysine; Protein Binding |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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See also
References
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