PMID:10417378

Citation	Ceriani, MF, Darlington, TK, Staknis, D, Más, P, Petti, AA, Weitz, CJ and Kay, SA (1999) Light-dependent sequestration of TIMELESS by CRYPTOCHROME. Science 285:553-6
Abstract	Most organisms have circadian clocks consisting of negative feedback loops of gene regulation that facilitate adaptation to cycles of light and darkness. In this study, CRYPTOCHROME (CRY), a protein involved in circadian photoperception in Drosophila, is shown to block the function of PERIOD/TIMELESS (PER/TIM) heterodimeric complexes in a light-dependent fashion. TIM degradation does not occur under these conditions; thus, TIM degradation is uncoupled from abrogation of its function by light. CRY and TIM are part of the same complex and directly interact in yeast in a light-dependent fashion. PER/TIM and CRY influence the subcellular distribution of these protein complexes, which reside primarily in the nucleus after the perception of a light signal. Thus, CRY acts as a circadian photoreceptor by directly interacting with core components of the circadian clock.
Links	PubMed
Keywords	Animals; Biological Clocks; Cell Line; Cell Nucleus/metabolism; Circadian Rhythm; Cryptochromes; Cytoplasm/metabolism; Darkness; Dimerization; Drosophila; Drosophila Proteins; Eye Proteins; Flavoproteins/genetics; Flavoproteins/metabolism; Green Fluorescent Proteins; Insect Proteins/genetics; Insect Proteins/metabolism; Light; Luminescent Proteins; Mutation; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Period Circadian Proteins; Photoreceptor Cells, Invertebrate; Receptors, G-Protein-Coupled; Recombinant Fusion Proteins/metabolism; Transfection; Yeasts/genetics; Yeasts/metabolism
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