GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:10400577
| Citation |
Masuda, S, Matsumoto, Y, Nagashima, KV, Shimada, K, Inoue, K, Bauer, CE and Matsuura, K (1999) Structural and functional analyses of photosynthetic regulatory genes regA and regB from Rhodovulum sulfidophilum, Roseobacter denitrificans, and Rhodobacter capsulatus. J. Bacteriol. 181:4205-15 |
|---|---|
| Abstract |
Genes coding for putative RegA, RegB, and SenC homologues were identified and characterized in the purple nonsulfur photosynthetic bacteria Rhodovulum sulfidophilum and Roseobacter denitrificans, species that demonstrate weak or no oxygen repression of photosystem synthesis. This additional sequence information was then used to perform a comparative analysis with previously sequenced RegA, RegB, and SenC homologues obtained from Rhodobacter capsulatus and Rhodobacter sphaeroides. These are photosynthetic bacteria that exhibit a high level of oxygen repression of photosystem synthesis controlled by the RegA-RegB two-component regulatory system. The response regulator, RegA, exhibits a remarkable 78.7 to 84.2% overall sequence identity, with total conservation within a putative helix-turn-helix DNA-binding motif. The RegB sensor kinase homologues also exhibit a high level of sequence conservation (55.9 to 61.5%) although these additional species give significantly different responses to oxygen. A Rhodovulum sulfidophilum mutant lacking regA or regB was constructed. These mutants produced smaller amounts of photopigments under aerobic and anaerobic conditions, indicating that the RegA-RegB regulon controls photosynthetic gene expression in this bacterium as it does as in Rhodobacter species. Rhodobacter capsulatus regA- or regB-deficient mutants recovered the synthesis of a photosynthetic apparatus that still retained regulation by oxygen tension when complemented with reg genes from Rhodovulum sulfidophilum and Roseobacter denitrificans. These results suggest that differential expression of photosynthetic genes in response to aerobic and anaerobic growth conditions is not the result of altered redox sensing by the sensor kinase protein, RegB. |
| Links | |
| Keywords |
Aerobiosis; Amino Acid Sequence; Anaerobiosis; Bacteria/genetics; Bacterial Physiological Phenomena; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; DNA, Bacterial/genetics; Gene Expression Regulation, Bacterial; Genes, Regulator; Molecular Sequence Data; Photosynthesis/genetics; Photosynthetic Reaction Center Complex Proteins/genetics; Photosynthetic Reaction Center Complex Proteins/metabolism; Protein Kinases; Rhodobacter capsulatus/genetics; Rhodobacter capsulatus/physiology; Sequence Analysis, DNA; Trans-Activators/genetics; Trans-Activators/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0018106: peptidyl-histidine phosphorylation |
ECO:0000247: |
UniProtKB:D5AKG6 UniProtKB:O82866 UniProtKB:Q3J6C1
|
P |
Analysis of the RegB sequence.The RegB homologues from all four photosynthetic bacteria are less highly conserved (55.9 to 61.5%) than are the RegA homologues (78.7 to 84.2%). However, sequence conservation is still quite high relative to that observed among other sensor kinases (covered in more detail in Discussion). Figure3 shows the amino acid sequence alignment of RegB of Rhodobacter capsulatus, Rhodovulum sulfidophilum, and Roseobacter denitrificans and of PrrB of Rhodobacter sphaeroides and ActS and RegS, which are thought to function as sensor kinase proteins responsible for phosphorylating ActR in R. meliloti and RegR in B. japonicum, respectively. |
complete | |||
See also
References
See Help:References for how to manage references in GONUTS.
