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PMID:10385520
| Citation |
Woods, LM, Hodges, CA, Baart, E, Baker, SM, Liskay, M and Hunt, PA (1999) Chromosomal influence on meiotic spindle assembly: abnormal meiosis I in female Mlh1 mutant mice. J. Cell Biol. 145:1395-406 |
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| Abstract |
In mouse oocytes, the first meiotic spindle is formed through the action of multiple microtubule organizing centers rather than a pair of centrosomes. Although the chromosomes are thought to play a major role in organizing the meiotic spindle, it remains unclear how a stable bipolar spindle is established. We have studied the formation of the first meiotic spindle in murine oocytes from mice homozygous for a targeted disruption of the DNA mismatch repair gene, Mlh1. In the absence of the MLH1 protein meiotic recombination is dramatically reduced and, as a result, the vast majority of chromosomes are present as unpaired univalents at the first meiotic division. The orientation of these univalent chromosomes at prometaphase suggests that they are unable to establish stable bipolar spindle attachments, presumably due to the inability to differentiate functional kinetochore domains on individual sister chromatids. In the presence of this aberrant chromosome behavior a stable first meiotic spindle is not formed, the spindle poles continue to elongate, and the vast majority of cells never initiate anaphase. These results suggest that, in female meiotic systems in which spindle formation is based on the action of multiple microtubule organizing centers, the chromosomes not only promote microtubule polymerization and organization but their attachment to opposite spindle poles acts to stabilize the forming spindle poles. |
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| Keywords |
Adaptor Proteins, Signal Transducing; Anaphase/genetics; Anaphase/physiology; Animals; Carrier Proteins; Centrosome/metabolism; Chromosome Segregation/genetics; Chromosome Segregation/physiology; Chromosomes/genetics; Chromosomes/physiology; Female; Homozygote; Kinetochores/physiology; Male; Meiosis/genetics; Meiosis/physiology; Mice; Mice, Knockout; Mitotic Spindle Apparatus/physiology; Mutation; Neoplasm Proteins/genetics; Neoplasm Proteins/physiology; Nuclear Proteins; Oocytes/physiology; Recombination, Genetic/genetics; Recombination, Genetic/physiology; Sex Chromosomes/genetics; Sex Chromosomes/physiology |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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