PMID:10373486

Citation	Das, K, Lewis, RY, Scherer, PE and Lisanti, MP (1999) The membrane-spanning domains of caveolins-1 and -2 mediate the formation of caveolin hetero-oligomers. Implications for the assembly of caveolae membranes in vivo. J. Biol. Chem. 274:18721-8
Abstract	The mammalian caveolin gene family consists of caveolins-1, -2, and -3. The expression of caveolin-3 is muscle-specific. In contrast, caveolins-1 and -2 are co-expressed, and they form a hetero-oligomeric complex in many cell types, with particularly high levels in adipocytes, endothelial cells, and fibroblasts. These caveolin hetero-oligomers are thought to represent the functional assembly units that drive caveolae formation in vivo. Here, we investigate the mechanism by which caveolins-1 and -2 form hetero-oligomers. We reconstituted this reciprocal interaction in vivo and in vitro using a variety of complementary approaches, including the generation of glutathione S-transferase fusion proteins and synthetic peptides. Taken together, our results indicate that the membrane-spanning domains of both caveolins-1 and -2 play a critical role in mediating their ability to interact with each other. This is the first demonstration that these unusual membrane-spanning regions found in the caveolin family play a specific role in protein-protein interactions.
Links	PubMed
Keywords	3T3 Cells; Amino Acid Sequence; Animals; COS Cells; Caveolin 1; Caveolin 2; Caveolins; Cell Membrane/chemistry; Cloning, Molecular; DNA, Complementary/chemistry; DNA, Complementary/isolation & purification; Epitope Mapping; Membrane Proteins/chemistry; Membrane Proteins/metabolism; Mice; Molecular Sequence Data; Open Reading Frames; Protein Conformation; Protein Structure, Secondary
edit table

Annotations

Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
edit table

References

See Help:References for how to manage references in GONUTS.