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PMID:10366593
| Citation |
Fisk, HA and Yaffe, MP (1999) A role for ubiquitination in mitochondrial inheritance in Saccharomyces cerevisiae. J. Cell Biol. 145:1199-208 |
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| Abstract |
The smm1 mutation suppresses defects in mitochondrial distribution and morphology caused by the mdm1-252 mutation in the yeast Saccharomyces cerevisiae. Cells harboring only the smm1 mutation themselves display temperature-sensitive growth and aberrant mitochondrial inheritance and morphology at the nonpermissive temperature. smm1 maps to RSP5, a gene encoding an essential ubiquitin-protein ligase. The smm1 defects are suppressed by overexpression of wild-type ubiquitin but not by overexpression of mutant ubiquitin in which lysine-63 is replaced by arginine. Furthermore, overexpression of this mutant ubiquitin perturbs mitochondrial distribution and morphology in wild-type cells. Site-directed mutagenesis revealed that the ubiquitin ligase activity of Rsp5p is essential for its function in mitochondrial inheritance. A second mutation, smm2, which also suppressed mdm1-252 defects, but did not cause aberrant mitochondrial distribution and morphology, mapped to BUL1, encoding a protein interacting with Rsp5p. These results indicate that protein ubiquitination mediated by Rsp5p plays an essential role in mitochondrial inheritance, and reveal a novel function for protein ubiquitination. |
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| Keywords |
Adaptor Proteins, Signal Transducing; Amino Acid Substitution; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cell Cycle Proteins; Endosomal Sorting Complexes Required for Transport; Fungal Proteins/genetics; Fungal Proteins/metabolism; Gene Expression; Genes, Essential/genetics; Genes, Fungal/genetics; Genes, Fungal/physiology; Genes, Suppressor/genetics; Genetic Complementation Test; Intermediate Filament Proteins; Ligases/genetics; Ligases/metabolism; Mitochondria/genetics; Mitochondria/physiology; Mutation; Phenotype; Protein Binding; Saccharomyces cerevisiae/cytology; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/growth & development; Saccharomyces cerevisiae Proteins; Suppression, Genetic; Temperature; Ubiquitin-Protein Ligase Complexes; Ubiquitin-Protein Ligases; Ubiquitins/genetics; Ubiquitins/metabolism; Ubiquitins/physiology |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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