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PMID:10344205
| Citation |
Sherson, S, Gy, I, Medd, J, Schmidt, R, Dean, C, Kreis, M, Lecharny, A and Cobbett, C (1999) The arabinose kinase, ARA1, gene of Arabidopsis is a novel member of the galactose kinase gene family. Plant Mol. Biol. 39:1003-12 |
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| Abstract |
The arabinose-sensitive ara1-1 mutant of Arabidopsis is deficient in arabinose kinase activity. A candidate for the ARA1 gene. ISA1, has been previously identified through the Arabidopsis genome sequencing initiative. Here we demonstrate that (1) the ARA1 gene coincides with ISA1 in a positional cloning strategy; (2) there are mutations in the ISA1 gene in both the ara1-1 mutant and an intragenic suppressor mutant; and (3) the ara1-1 and suppressor mutant phenotypes can be complemented by the expression of the ISA1 cDNA in transgenic plants. Together these observations confirm that ISA1 is the ARA1 gene. ARA1 is a member of the galactose kinase family of genes and represents a new substrate specificity among this and other families of sugar kinases. A second gene with similarities to members of the galactose kinase gene family has been identified in the EST database. A 1.8 kb cDNA contained an open reading-frame predicted to encode a 496 amino acid polypeptide. The GAL1 cDNA was expressed in a galK mutant of Escherichia coli and in vitro assays of extracts of the strain expressing GAL1 confirmed that the cDNA encodes a galactose kinase activity. Both GAL1 and ARA1 cross-hybridise at low stringency to other sequences suggesting the presence of additional members of the galactose kinase gene family. |
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| Keywords |
Amino Acid Sequence; Arabidopsis/chemistry; Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis Proteins; Chromosome Walking; Cloning, Molecular; DNA, Complementary/chemistry; DNA, Complementary/genetics; DNA, Plant/chemistry; DNA, Plant/genetics; Galactokinase/genetics; Genes, Plant/genetics; Genetic Complementation Test; Molecular Sequence Data; Phosphotransferases (Alcohol Group Acceptor)/genetics; Physical Chromosome Mapping; Sequence Analysis, DNA; Sequence Homology, Amino Acid |
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Significance
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| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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