PMID:10200548

Citation	Gillardon, F, Moll, I, Meyer, M and Michaelidis, TM (1999) Alterations in cell death and cell cycle progression in the UV-irradiated epidermis of bcl-2-deficient mice. Cell Death Differ. 6:55-60
Abstract	The effect of bcl-2 gene ablation on epidermal cell death induced by UV-B irradiation was investigated in mice. Exposure of depilated back skin of bcl-2-/- mice to 0.5 J/cm2 UV-B caused a prolonged increase in the number of epidermal cells showing nuclear DNA fragmentation compared to wild-type littermates. Consistently, skin explants from bcl-2-deficient mice exhibited a higher number of sunburn cells per cm epidermis (16.6+/-2.1 vs 7.0+/-1.5) following exposure to 0.1 J/cm2 UV-B in vitro. Furthermore, UV irradiation failed to increase pre-melanosomes in skin explants from mutant animals, and primary menalocyte cultures derived from bcl-2 null mutants were highly susceptible to UV-induced cell death compared to cultures from wild-type littermates. An accelerated reappearance of proliferating cells, showing nuclear immunoreactivity for Ki-67 and c-Fos, was observed in the UV-irradiated epidermis of bcl-2-deficient mice. Taken together, these findings suggest that effects of UV radiation on epidermal cell death and cell cycle progression are influenced by survival-promoting Bcl-2.
Links	PubMed Online version:10.1038/sj.cdd.4400455
Keywords	Animals; Apoptosis/genetics; Apoptosis/radiation effects; Cell Count/radiation effects; Cell Cycle/genetics; Cell Cycle/radiation effects; DNA Fragmentation/genetics; DNA Fragmentation/radiation effects; Epidermis/radiation effects; Immunohistochemistry; In Situ Nick-End Labeling; Ki-67 Antigen/metabolism; Melanocytes/radiation effects; Mice; Mice, Knockout; Proto-Oncogene Proteins c-bcl-2/deficiency; Proto-Oncogene Proteins c-bcl-2/genetics; Ultraviolet Rays
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