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PMID:10197531
| Citation |
Brückner, K, Pablo Labrador, J, Scheiffele, P, Herb, A, Seeburg, PH and Klein, R (1999) EphrinB ligands recruit GRIP family PDZ adaptor proteins into raft membrane microdomains. Neuron 22:511-24 |
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| Abstract |
Transmembrane ephrinB proteins have important functions during embryonic patterning as ligands for Eph receptor tyrosine kinases and presumably as signal-transducing receptor-like molecules. Consistent with "reverse" signaling, ephrinB1 is localized in sphingo-lipid/cholesterol-enriched raft microdomains, platforms for the localized concentration and activation of signaling molecules. Glutamate receptor-interacting protein (GRIP) and a highly related protein, which we have termed GRIP2, are recruited into these rafts through association with the C-terminal PDZ target site of ephrinB1. Stimulation of ephrinB1 with soluble EphB2 receptor ectodomain causes the formation of large raft patches that also contain GRIP proteins. Moreover, a GRIP-associated serine/threonine kinase activity is recruited into ephrinB1-GRIP complexes. Our findings suggest that GRIP proteins provide a scaffold for the assembly of a multiprotein signaling complex downstream of ephrinB ligands. |
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| Keywords |
Adaptor Proteins, Signal Transducing; Amino Acid Sequence; Animals; Blotting, Western; Brain/embryology; Brain/metabolism; Carrier Proteins/genetics; Carrier Proteins/metabolism; Carrier Proteins/physiology; Cell Line; Cell Membrane/metabolism; Cell Membrane/ultrastructure; Ephrin-B1; Gene Deletion; In Situ Hybridization; Ligands; Membrane Proteins/metabolism; Membrane Proteins/physiology; Mice; Molecular Sequence Data; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/metabolism; Nerve Tissue Proteins/physiology; Neurons/metabolism; Neurons/ultrastructure; Organ Specificity; Phosphoamino Acids/metabolism; Precipitin Tests; Protein-Serine-Threonine Kinases/metabolism; Rats |
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