PMID:10193316

Citation	Boot-Handford, RP, Michaelidis, TM, Hillarby, MC, Zambelli, A, Denton, J, Hoyland, JA, Freemont, AJ, Grant, ME and Wallis, GA (1998) The bcl-2 knockout mouse exhibits marked changes in osteoblast phenotype and collagen deposition in bone as well as a mild growth plate phenotype. Int J Exp Pathol 79:329-35
Abstract	Histological examination of long bones from 1-day-old bcl-2 knockout and age-matched control mice revealed no obvious differences in length of bone, growth plate architecture or stage of endochondral ossification. In 35-day-old bcl-2 knockout mice that are growth retarded or 'dwarfed'. the proliferative zone of the growth plate appeared slightly thinner and the secondary centres of ossification less well developed than their age-matched wild-type controls. The most marked histological effects of bcl-2 ablation were on osteoblasts and bone. 35-day-old knockout mouse bones exhibited far greater numbers of osteoblasts than controls and the osteoblasts had a cuboidal phenotype in comparison with the normal flattened cell appearance. In addition, the collagen deposited by the osteoblasts in the bcl-2 knockout mouse bone was disorganized in comparison with control tissue and had a pseudo-woven appearance. The results suggest an important role for Bcl-2 in controlling osteoblast phenotype and bone deposition in vivo.
Links	PubMed PMC3220196
Keywords	Animals; Bone and Bones/metabolism; Bone and Bones/pathology; Collagen/metabolism; Dwarfism/genetics; Dwarfism/metabolism; Dwarfism/pathology; Genes, bcl-2; Growth Plate/pathology; Immunoenzyme Techniques; Mice; Mice, Knockout; Osteoblasts/pathology; Osteoblasts/physiology; Phenotype
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