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Callaghan, MJ, Russell, AJ, Woollatt, E, Sutherland, GR, Sutherland, RL and Watts, CK (1998) Identification of a human HECT family protein with homology to the Drosophila tumor suppressor gene hyperplastic discs. Oncogene 17:3479-91
Use of the differential display technique to isolate progestin-regulated genes in T-47D human breast cancer cells led to identification of a novel gene, EDD. The cDNA sequence contains a 2799 amino acid open reading frame sharing 40% identity with the predicted 2894 amino acid product of the Drosophila melanogaster tumor suppressor gene hyperplastic discs, while the carboxy-terminal 889 amino acids show 96% identity to a rat 100 kDa HECT domain protein. EDD mRNA was progestin-induced in T-47D cells and was highly abundant in testes and expressed at moderately high levels in other tissues, suggesting a broad role for EDD. Anti-EDD antibodies immunoprecipitated an approximately 300 kDa protein from T-47D cell lysates. HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. EDD is likely to function as an E3 as in vitro translated protein bound ubiquitin reversibly through a conserved HECT domain cysteine residue. EDD was localized by FISH to chromosome 8q22, a locus disrupted in a variety of cancers. Given the homology between EDD and the hyperplastic discs protein, which is required for control of imaginal disc growth in Drosophila, EDD potentially has a role in regulation of cell proliferation or differentiation.
Amino Acid Sequence; Animals; Blotting, Northern; Chromosomes, Human, Pair 8; Cloning, Molecular; Drosophila melanogaster/genetics; Genetic Techniques; Helminth Proteins/genetics; Humans; Ligases/genetics; Ligases/metabolism; Male; Molecular Sequence Data; Peptide Synthases; Pregnenediones/pharmacology; Proteins/genetics; Proteins/metabolism; Rats; Sequence Homology, Amino Acid; Ubiquitin-Protein Ligases; Ubiquitins/metabolism
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