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MYCSM:DHA

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Species (Taxon ID) Mycobacterium smegmatis. (1772)
Gene Name(s) ald
Protein Name(s) Alanine dehydrogenase
External Links
UniProt Q8KY18
EMBL AF304867
RefSeq YP_006567359.1
YP_886996.1
ProteinModelPortal Q8KY18
SMR Q8KY18
GeneID 4534068
KEGG msm:MSMEG_2659
PATRIC 18077746
KO K00259
OrthoDB EOG6Z6FTG
UniPathway UPA00527
GO GO:0005829
GO:0000286
GO:0046872
GO:0000166
GO:0006524
GO:0042853
Gene3D 3.40.50.720
InterPro IPR008141
IPR008143
IPR008142
IPR007886
IPR007698
IPR016040
Pfam PF01262
PF05222
PIRSF PIRSF000183
SMART SM01002
SM01003
TIGRFAMs TIGR00518
PROSITE PS00836
PS00837

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status

enables

GO:0000286

alanine dehydrogenase activity

PMID:12193615[1]

ECO:0000315

mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

GO:0000286

alanine dehydrogenase activity

PMID:12193615[1]

ECO:0000315

F

Figure 4c: Displays the results from the analysis of L-alanine dehydrogenase activities in M. smegmatis wild type, mutant, and recombinant strains. The wild type strain (mc2155) with added L-alanine has the highest specific activity levels, compared to ald mutant and recombinant strains.

complete
CACAO 9691

involved_in

GO:0006524

alanine catabolic process

GO_REF:0000024

ECO:0000250

sequence similarity evidence used in manual assertion

UniProtKB:P9WQB1

P

Seeded From UniProt

complete

GO:0000286

alanine dehydrogenase activity

PMID:12193615[1]

ECO:0000315

F

Figure 6b: Shows the utilization of nitrogen sources by M. smegmatis wild-type, ald null mutant, and complemented strains. Exponentially of each strain were harvested, washed, and inoculated into minimal medium containing L-alanine as the sole nitrogen source. More viable cells were produced in the wild type strain compared to mutant and complemented strains. This is because alanine dehydrogenase catalyzes a reaction that generates nitrogen from L-alanine.

complete
CACAO 9692

part_of

GO:0005829

cytosol

GO_REF:0000024

ECO:0000250

sequence similarity evidence used in manual assertion

UniProtKB:P9WQB1

C

Seeded From UniProt

complete

enables

GO:0000286

alanine dehydrogenase activity

PMID:12193615[1]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0000286

alanine dehydrogenase activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR008141

F

Seeded From UniProt

complete

enables

GO:0016491

oxidoreductase activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR008142
InterPro:IPR008143

F

Seeded From UniProt

complete

involved_in

GO:0042853

L-alanine catabolic process

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR008141

P

Seeded From UniProt

complete

involved_in

GO:0055114

oxidation-reduction process

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR008142
InterPro:IPR008143

P

Seeded From UniProt

complete

enables

GO:0000286

alanine dehydrogenase activity

GO_REF:0000003

ECO:0000501

evidence used in automatic assertion

EC:1.4.1.1

F

Seeded From UniProt

complete

enables

GO:0016491

oxidoreductase activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0560

F

Seeded From UniProt

complete

involved_in

GO:0055114

oxidation-reduction process

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0560

P

Seeded From UniProt

complete

enables

GO:0000166

nucleotide binding

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0547

F

Seeded From UniProt

complete

part_of

GO:0005737

cytoplasm

GO_REF:0000037
GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0963
UniProtKB-SubCell:SL-0086

C

Seeded From UniProt

complete

involved_in

GO:0042853

L-alanine catabolic process

GO_REF:0000041

ECO:0000322

imported manually asserted information used in automatic assertion

UniPathway:UPA00527

P

Seeded From UniProt

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. 1.0 1.1 1.2 1.3 Feng, Z et al. (2002) Mycobacterium smegmatis L-alanine dehydrogenase (Ald) is required for proficient utilization of alanine as a sole nitrogen source and sustained anaerobic growth. J. Bacteriol. 184 5001-10 PubMed GONUTS page