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MOUSE:MFN1
Contents
| Species (Taxon ID) | Mus musculus (Mouse). (10090) | |
| Gene Name(s) | Mfn1 | |
| Protein Name(s) | Mitofusin-1
Transmembrane GTPase MFN1 | |
| External Links | ||
| UniProt | Q811U4 | |
| EMBL | AY174062 AK009490 AK018181 AK141611 BC002133 BC047050 BC056641 | |
| CCDS | CCDS17296.1 | |
| RefSeq | NP_077162.2 | |
| UniGene | Mm.290414 | |
| PDB | 1T3J | |
| PDBsum | 1T3J | |
| ProteinModelPortal | Q811U4 | |
| SMR | Q811U4 | |
| BioGrid | 212170 | |
| DIP | DIP-60969N | |
| IntAct | Q811U4 | |
| STRING | 10090.ENSMUSP00000088801 | |
| iPTMnet | Q811U4 | |
| PhosphoSitePlus | Q811U4 | |
| EPD | Q811U4 | |
| MaxQB | Q811U4 | |
| PaxDb | Q811U4 | |
| PeptideAtlas | Q811U4 | |
| PRIDE | Q811U4 | |
| Ensembl | ENSMUST00000091257 ENSMUST00000118286 | |
| GeneID | 67414 | |
| KEGG | mmu:67414 | |
| UCSC | uc008owi.2 | |
| CTD | 55669 | |
| MGI | MGI:1914664 | |
| eggNOG | KOG0448 COG0699 | |
| GeneTree | ENSGT00390000013727 | |
| HOVERGEN | HBG052465 | |
| InParanoid | Q811U4 | |
| KO | K06030 | |
| OMA | AMTDEIC | |
| OrthoDB | EOG091G02ES | |
| TreeFam | TF314289 | |
| Reactome | [www.reactome.org/content/detail/R-MMU-5205685 R-MMU-5205685] | |
| ChiTaRS | Mfn1 | |
| EvolutionaryTrace | Q811U4 | |
| PRO | PR:Q811U4 | |
| Proteomes | UP000000589 | |
| Bgee | ENSMUSG00000027668 | |
| CleanEx | MM_MFN1 | |
| ExpressionAtlas | Q811U4 | |
| Genevisible | Q811U4 | |
| GO | GO:0016021 GO:0031306 GO:0005739 GO:0005525 GO:0003924 GO:0008053 GO:0051646 GO:0007275 | |
| Gene3D | 3.40.50.300 | |
| InterPro | IPR022812 IPR006884 IPR030381 IPR027088 IPR027417 | |
| PANTHER | PTHR10465:SF7 | |
| Pfam | PF00350 PF04799 | |
| SUPFAM | SSF52540 | |
| PROSITE | PS51718 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0008053 |
mitochondrial fusion |
ECO:0000315 |
P |
Mfn1 clearly demonstrates positive regulation amount of mitochondrial fusion, as seen and inferred from Table 1 Uniprot Nomenclature: Mitofusin-1 (MFN1_MOUSE) Uniprot ID: Q811U4 Organism: Mus musculus (Mouse) |
complete | |||||
| GO:0010918 |
positive regulation of mitochondrial membrane potential |
ECO:0000316 |
|
P |
MFn1 reveals that it is a positive regulator of mitochondrial membrane potential as loss of membrane potential was reversible when MFN-null cells when they began to over express Mfn1. Figure 4A-C Uniprot Nomenclature: Mitofusin-1 (MFN1_MOUSE) Organism: Mus musculus (Mouse) "Consistent with our previous study (3), we found that both Mfn1-null and Mfn2-null cells have mild heterogeneity in mitochondrial ΔΨ.2 Most Mfn1-null cells showed some mitochondria with weak MitoTracker Red staining, but this was restricted to only a few mitochondria (1–10/cell). With Mfn2-null cells, only a minority of cells showed heterogeneity in MitoTracker staining, and again this defect was restricted to a few mitochondria. In contrast, 100% of Mfn-dm cells showed widespread heterogeneity of mitochondrial ΔΨ, with a large fraction of the mitochondrial population failing to stain well with MitoTracker Red (Fig. 4, A–C). The loss of mitochondrial ΔΨ was reversible with complete restoration of ΔΨ in Mfn-null cells overexpressing Mfn1.2 Depletion of OPA1 by RNAi similarly led to wide-spread loss of mitochondrial ΔΨ (Fig. 4, D–F). Cells overexpressing OPA1 showed no ΔΨ defects (Fig. 4, G–I). Taken together with the PEG fusion data, these results indicate that the severity of mitochondrial ΔΨ loss correlates with the severity of fusion deficiency. " |
complete | ||||
| GO:0030307 |
positive regulation of cell growth |
ECO:0000315 |
P |
Mfn1 is needed for cell growth, as seen in Supplemental Fig. S1 and as Mfn2-null cells depleted of Mfn1 by shRNA grew to only 18% of the parental Mfn2-null control. Figure 3B also showed growth rescue by reintroduction of Mfn1. Uniprot Nomenclature: Mitofusin-1 (MFN1_MOUSE) Uniprot ID: Q811U4 Organism: Mus musculus (Mouse) |
complete | |||||
| GO:0070584 |
mitochondrion morphogenesis |
ECO:0000316 |
|
P |
Mfn-dm (double mutant) cells revealed the necessity of Mfn1 and Mfn2 for mitochondrial morphology, Therefore through this double mutant, the reliance of Mfn2 and Mfn1 for Mitochondrial morphology is confirmed. Seen in FIG. 1. Mitofusin-2 and Mitofusin-1 Mus musculus (Mouse) |
complete | ||||
| GO:0008053 |
mitochondrial fusion |
ECO:0000316 |
|
P |
Mfn-dm (double mutant) cells had 0% mitochondrial fusion, as seen on Table I. Table 1 and FIG. 2 illustrate the importance of both Mfn1 and Mfn2 for mitochondrial fusion. Mitofusin-2 and Mitofusin-1 Mus musculus (Mouse) |
complete | ||||
| GO:0016049 |
cell growth |
ECO:0000316 |
|
P |
Mfn-dm (double mutant) cells showed growth defects, as seen on FIG. 3. Thus, Mfn1 and Mfn2 are required for appropriate cell growth. Mitofusin-2 and Mitofusin-1 Mus musculus (Mouse) |
complete | ||||
| GO:0009060 |
aerobic respiration |
ECO:0000316 |
|
P |
Mfn-dm (double mutant) showed respiratory defects, as seen on FIG. 5. These defects were reversible, as seen in Mfn1 and Mfn2, thus showing the importance of Mfn1 and 2 for aerobic respiration. Mitofusin-2 and Mitofusin-1 Mus musculus (Mouse) |
complete | ||||
|
part_of |
GO:0098799 |
outer mitochondrial membrane protein complex |
ECO:0000250 |
sequence similarity evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0031307 |
integral component of mitochondrial outer membrane |
ECO:0000250 |
sequence similarity evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003924 |
GTPase activity |
ECO:0000250 |
sequence similarity evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:1990613 |
mitochondrial membrane fusion |
ECO:0000250 |
sequence similarity evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0051646 |
mitochondrion localization |
ECO:0000250 |
sequence similarity evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0046039 |
GTP metabolic process |
ECO:0000250 |
sequence similarity evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0008053 |
mitochondrial fusion |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0051646 |
mitochondrion localization |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN000825684 |
P |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0031306 |
intrinsic component of mitochondrial outer membrane |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN000825684 |
C |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0008053 |
mitochondrial fusion |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
FB:FBgn0011596 |
P |
Seeded From UniProt |
complete | ||
|
enables |
GO:0003924 |
GTPase activity |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN000049197 |
F |
Seeded From UniProt |
complete | ||
|
acts_upstream_of_or_within |
GO:1990613 |
mitochondrial membrane fusion |
ECO:0000266 |
sequence orthology evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0098799 |
outer mitochondrial membrane protein complex |
ECO:0000266 |
sequence orthology evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
acts_upstream_of_or_within |
GO:0090258 |
negative regulation of mitochondrial fission |
ECO:0000266 |
sequence orthology evidence used in manual assertion |
RGD:621460 |
P |
Seeded From UniProt |
complete | ||
|
acts_upstream_of_or_within |
GO:0070584 |
mitochondrion morphogenesis |
ECO:0000266 |
sequence orthology evidence used in manual assertion |
RGD:621460 |
P |
Seeded From UniProt |
complete | ||
|
acts_upstream_of_or_within |
GO:0061003 |
positive regulation of dendritic spine morphogenesis |
ECO:0000266 |
sequence orthology evidence used in manual assertion |
RGD:621460 |
P |
Seeded From UniProt |
complete | ||
|
acts_upstream_of_or_within |
GO:0051646 |
mitochondrion localization |
ECO:0000266 |
sequence orthology evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
acts_upstream_of_or_within |
GO:0048312 |
intracellular distribution of mitochondria |
ECO:0000266 |
sequence orthology evidence used in manual assertion |
RGD:621460 |
P |
Seeded From UniProt |
complete | ||
|
acts_upstream_of_or_within |
GO:0046039 |
GTP metabolic process |
ECO:0000266 |
sequence orthology evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0042803 |
protein homodimerization activity |
ECO:0000266 |
sequence orthology evidence used in manual assertion |
RGD:621460 |
F |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0031307 |
integral component of mitochondrial outer membrane |
ECO:0000266 |
sequence orthology evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0016021 |
integral component of membrane |
ECO:0000266 |
sequence orthology evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
acts_upstream_of_or_within |
GO:0010636 |
positive regulation of mitochondrial fusion |
ECO:0000266 |
sequence orthology evidence used in manual assertion |
RGD:621460 |
P |
Seeded From UniProt |
complete | ||
|
acts_upstream_of_or_within |
GO:0008053 |
mitochondrial fusion |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
MGI:MGI:2450304 |
P |
Seeded From UniProt |
complete | ||
|
acts_upstream_of_or_within |
GO:0008053 |
mitochondrial fusion |
ECO:0000266 |
sequence orthology evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005743 |
mitochondrial inner membrane |
ECO:0000266 |
sequence orthology evidence used in manual assertion |
RGD:621460 |
C |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005741 |
mitochondrial outer membrane |
ECO:0000266 |
sequence orthology evidence used in manual assertion |
RGD:621460 |
C |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005739 |
mitochondrion |
ECO:0007005 |
high throughput direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005739 |
mitochondrion |
ECO:0000266 |
sequence orthology evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003924 |
GTPase activity |
ECO:0000266 |
sequence orthology evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:1990613 |
mitochondrial membrane fusion |
ECO:0000265 |
sequence orthology evidence used in automatic assertion |
UniProtKB:Q8IWA4 |
P |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0098799 |
outer mitochondrial membrane protein complex |
ECO:0000265 |
sequence orthology evidence used in automatic assertion |
UniProtKB:Q8IWA4 |
C |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0051646 |
mitochondrion localization |
ECO:0000265 |
sequence orthology evidence used in automatic assertion |
UniProtKB:Q8IWA4 |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0046039 |
GTP metabolic process |
ECO:0000265 |
sequence orthology evidence used in automatic assertion |
UniProtKB:Q8IWA4 |
P |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0031307 |
integral component of mitochondrial outer membrane |
ECO:0000265 |
sequence orthology evidence used in automatic assertion |
UniProtKB:Q8IWA4 |
C |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0016021 |
integral component of membrane |
ECO:0000265 |
sequence orthology evidence used in automatic assertion |
UniProtKB:Q8IWA4 |
C |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0008053 |
mitochondrial fusion |
ECO:0000265 |
sequence orthology evidence used in automatic assertion |
UniProtKB:Q8IWA4 |
P |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005739 |
mitochondrion |
ECO:0000265 |
sequence orthology evidence used in automatic assertion |
UniProtKB:Q8IWA4 |
C |
Seeded From UniProt |
complete | ||
|
enables |
GO:0003924 |
GTPase activity |
ECO:0000265 |
sequence orthology evidence used in automatic assertion |
UniProtKB:Q8IWA4 |
F |
Seeded From UniProt |
complete | ||
|
enables |
GO:0003924 |
GTPase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0005525 |
GTP binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005741 |
mitochondrial outer membrane |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0008053 |
mitochondrial fusion |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0016021 |
integral component of membrane |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0007275 |
multicellular organism development |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0000166 |
nucleotide binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016787 |
hydrolase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005739 |
mitochondrion |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005741 |
mitochondrial outer membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0005525 |
GTP binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0016021 |
integral component of membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0016020 |
membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Chen, H et al. (2005) Disruption of fusion results in mitochondrial heterogeneity and dysfunction. J. Biol. Chem. 280 26185-92 PubMed GONUTS page
- ↑ Yue, W et al. (2014) A small natural molecule promotes mitochondrial fusion through inhibition of the deubiquitinase USP30. Cell Res. 24 482-96 PubMed GONUTS page
- ↑ 3.0 3.1 3.2 3.3 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page
- ↑ Chen, H et al. (2003) Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development. J. Cell Biol. 160 189-200 PubMed GONUTS page
- ↑ Pagliarini, DJ et al. (2008) A mitochondrial protein compendium elucidates complex I disease biology. Cell 134 112-23 PubMed GONUTS page
i
m
- Mammalia
- GO:0016020 ! membrane
- Metazoa
- GO:0005741 ! mitochondrial outer membrane
- GO:0008053 ! mitochondrial fusion
- GO:0005743 ! mitochondrial inner membrane
- GO:1990613 ! mitochondrial membrane fusion
- GO:0051646 ! mitochondrion localization
- GO:0005739 ! mitochondrion
- GO:0007275 ! multicellular organism development
- Muridae
- Murinae
- Muroidea
- Mus
- Mus musculus
o
p
r
