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LACCA:A0A0E2LWA2

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Species (Taxon ID) Lactobacillus casei 5b. (1378069)
Gene Name(s) No Information Provided.
Protein Name(s) Lysin (ECO:0000313 with EMBL:ERN48936.1)
External Links
UniProt A0A0E2LWA2
EMBL AUYM01000070
RefSeq WP_012491268.1
EnsemblBacteria ERN48936
Proteomes UP000017134
InterPro IPR008044
Pfam PF05382

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
GO:0004175

endopeptidase activity

PMID:23733182[1]

ECO:0000314

F

fig. 3B and Table 3 show the hydrolytic specificity of the recombinant pure Lc-Lys-2 on Lactobacillus casei-purified Peptidoglycan (PG). The PG fragment obtained by digestion with Lc-Lys-2 was separated by RP-HPLC and analyzed by MALDI-TOF mass spectrometry. The HPLC profile reveal the hydrolytic specificities for Lc-Lys-2. MS analysis of the major peaks and comparison of the obtained masses with the reference L. casei PG structure enabled identification of peptides generated by Lc-Lys-2 (Table 3) and to deduce the cleavage specificity (Fig. 3D). Lc-Lys-2 has a gamma-D-glutamyl-L-lysyl endopeptidase specificity.

complete
CACAO 12620

Notes

References

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  1. Regulski, K et al. (2013) A novel type of peptidoglycan-binding domain highly specific for amidated D-Asp cross-bridge, identified in Lactobacillus casei bacteriophage endolysins. J. Biol. Chem. 288 20416-26 PubMed GONUTS page