HCMVM:DEN

Species (Taxon ID)	Human cytomegalovirus (strain Merlin) (HHV-5) (Human herpesvirus 5). (295027)
Gene Name(s)	UL48
Protein Name(s)	Deneddylase Large tegument protein LTP
External Links
UniProt	Q6SW84
EMBL	AY446894
RefSeq	YP_081506.1
ProteinModelPortal	Q6SW84
GeneID	3077504
Proteomes	UP000000938
GO	GO:0030430 GO:0042025 GO:0019033 GO:0008234 GO:0039648
InterPro	IPR006928
Pfam	PF04843
PROSITE	PS51521

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
	GO:0004843	ubiquitin-specific protease activity	PMID:PMC2786740	ECO:0000315			F		Figure 1 shows that HCMV UL48 has deubiquitinase activity. A UL48 wild type was isolated as well as two point mutants that had amino acid substitutions in the presumed active site. They also generated several fusion proteins. All of these had GST at their amino ends and were tagged. One of these fusion proteins contained ubiquitin (Ub) . Figure 1C shows that when expressed UL48 was incubated with the fusion proteins, it cleaved GST-Ub-HA to produce GST-Ub, but it did not cleave any of the other proteins. This was measured using gel electrophoresis. When the two active-site UL48 mutatns were tested in figure 1D, not activity was detected, showing that their ubiquitinase activity was impaired. Protein staining in figure 1E showed that there were comparable amounts of the WT and mutant UL48 proteins in each assay, so the differences in activity can't be attributed in differences in amount of protein present.	complete CACAO 8006
enables	GO:0008234	cysteine-type peptidase activity	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR034702	F		Seeded From UniProt	complete
enables	GO:0036459	thiol-dependent ubiquitinyl hydrolase activity	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR034702	F		Seeded From UniProt	complete
enables	GO:0036459	thiol-dependent ubiquitinyl hydrolase activity	GO_REF:0000003	ECO:0000501	evidence used in automatic assertion	EC:3.4.19.12	F		Seeded From UniProt	complete
part_of	GO:0019033	viral tegument	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000410382	C		Seeded From UniProt	complete
involved_in	GO:0039648	modulation by virus of host protein ubiquitination	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000410382	P		Seeded From UniProt	complete
enables	GO:0036459	thiol-dependent ubiquitinyl hydrolase activity	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000410382	F		Seeded From UniProt	complete
part_of	GO:0030430	host cell cytoplasm	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000410382	C		Seeded From UniProt	complete
part_of	GO:0042025	host cell nucleus	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000410382	C		Seeded From UniProt	complete
involved_in	GO:0016032	viral process	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0945	P		Seeded From UniProt	complete
part_of	GO:0042025	host cell nucleus	GO_REF:0000037 GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1048 UniProtKB-SubCell:SL-0414	C		Seeded From UniProt	complete
involved_in	GO:0039648	modulation by virus of host protein ubiquitination	GO_REF:0000037 GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1127 UniProtKB-KW:KW-1130	P		Seeded From UniProt	complete
involved_in	GO:0006508	proteolysis	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0645	P		Seeded From UniProt	complete
part_of	GO:0019033	viral tegument	GO_REF:0000037 GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0920 UniProtKB-SubCell:SL-0273	C		Seeded From UniProt	complete
enables	GO:0016787	hydrolase activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0378	F		Seeded From UniProt	complete
enables	GO:0008233	peptidase activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0645	F		Seeded From UniProt	complete
part_of	GO:0030430	host cell cytoplasm	GO_REF:0000037 GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1035 UniProtKB-SubCell:SL-0381	C		Seeded From UniProt	complete
enables	GO:0008234	cysteine-type peptidase activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0788	F		Seeded From UniProt	complete
part_of	GO:0019012	virion	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0946	C		Seeded From UniProt	complete
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