HCMVM:AN

Species (Taxon ID)	Human cytomegalovirus (strain Merlin) (HHV-5) (Human herpesvirus 5). (295027)
Gene Name(s)	UL98
Protein Name(s)	Alkaline nuclease
External Links
UniProt	F5HF49
EMBL	AY446894
RefSeq	YP_081545.1
ProteinModelPortal	F5HF49
GeneID	3077527
Proteomes	UP000000938
GO	GO:0042025 GO:0003677 GO:0004520 GO:0004529 GO:0000737 GO:0000738
Gene3D	3.90.320.10
InterPro	IPR011604 IPR001616 IPR011335
Pfam	PF01771
PRINTS	PR00924
SUPFAM	SSF52980

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
	GO:0004529	exodeoxyribonuclease activity	PMID:21900421	ECO:0000314			F		Figure 2 shows the results demonstrating UL98's exonuclease activity. There is a wild type (WT) UL98 strain and five mutants in which alanine was used to substitute another residue within the gene. The expressed proteins were purified and labelled. They were then combined with 14C-labelled DNA. The amount of cleaved DNA was measured over time. Figure 2a shows the significantly different cleavage rates between the WT gene and the R164A mutant, whose function was greatly impaired. Figure 2b show the total cleavage done by the WT and all the mutants over the course of an hour. The WT gene product exhibited significantly more exonuclease activity than the UL98-impaired mutants.	complete CACAO 7973
	GO:0004520	endodeoxyribonuclease activity	PMID:21900421	ECO:0000314			F		Figure 3 shows the results demonstrating UL98's endonuclease activity. There is a wild type (WT) UL98 strain and five mutants in which alanine was used to substitute another residue within the gene. The expressed proteins were purified and labelled. In figure 3a, the WT and mutants were incubated with closed-circular plasmid DNA to see if the gene products would convert it to open-circular and linear forms using endonuclease activity. Following incubation, gel electrophoresis measured the leftover DNA products. The WT and one of the mutants were able to degrade the entire DNA substrate by nicking the supercoiled substrate and processing the products into smaller fragments not visible on the gel. Other mutants were only able to nick the supercoiled DNA, but not digest anything else. In figure 3b, the WT and mutants were incubated with a ssDNA substrate that had a 3' quencher and a 5' fluorophore. Endonucleolytic cleavage would result in detectable fluorescence. Over time, more protein was added to the reaction in increments. The WT and one of the mutants produced significantly more fluorescence than the rest of the mutants. This indicates that those mutants suffered from impaired endonuclease function. The R164A mutant had a slightly reduced endonuclease activity compaired to the WT.	complete CACAO 7974
enables	GO:0004529	exodeoxyribonuclease activity	PMID:21900421	ECO:0000314	direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
enables	GO:0004520	endodeoxyribonuclease activity	PMID:21900421	ECO:0000314	direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
involved_in	GO:0006259	DNA metabolic process	GO_REF:0000108	ECO:0000364	evidence based on logical inference from manual annotation used in automatic assertion	GO:0004529	P		Seeded From UniProt	complete
enables	GO:0003677	DNA binding	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR001616	F		Seeded From UniProt	complete
enables	GO:0004527	exonuclease activity	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR001616	F		Seeded From UniProt	complete
involved_in	GO:0016032	viral process	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000281433	P		Seeded From UniProt	complete
involved_in	GO:0090305	nucleic acid phosphodiester bond hydrolysis	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000281433	P		Seeded From UniProt	complete
part_of	GO:0030430	host cell cytoplasm	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000281433	C		Seeded From UniProt	complete
enables	GO:0004519	endonuclease activity	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000281433	F		Seeded From UniProt	complete
part_of	GO:0042025	host cell nucleus	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000281433	C		Seeded From UniProt	complete
enables	GO:0003677	DNA binding	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000281433	F		Seeded From UniProt	complete
enables	GO:0004518	nuclease activity	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000281433	F		Seeded From UniProt	complete
enables	GO:0004527	exonuclease activity	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000281433	F		Seeded From UniProt	complete
part_of	GO:0030430	host cell cytoplasm	GO_REF:0000037 GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1035 UniProtKB-SubCell:SL-0381	C		Seeded From UniProt	complete
enables	GO:0004527	exonuclease activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0269	F		Seeded From UniProt	complete
enables	GO:0016787	hydrolase activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0378	F		Seeded From UniProt	complete
enables	GO:0004519	endonuclease activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0255	F		Seeded From UniProt	complete
enables	GO:0004518	nuclease activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0540	F		Seeded From UniProt	complete
involved_in	GO:0016032	viral process	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0945	P		Seeded From UniProt	complete
part_of	GO:0042025	host cell nucleus	GO_REF:0000037 GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1048 UniProtKB-SubCell:SL-0414	C		Seeded From UniProt	complete
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References

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