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ECOLI:TRUB

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Species (Taxon ID) Escherichia coli (strain K12). (83333)
Gene Name(s) truB (synonyms: yhbA)
Protein Name(s) tRNA pseudouridine synthase B

Protein p35 tRNA pseudouridine(55) synthase Psi55 synthase tRNA pseudouridylate synthase tRNA-uridine isomerase

External Links
UniProt P60340
EMBL X13270
X13775
U18997
U00096
AP009048
S79856
PIR S01916
RefSeq NP_417635.1
YP_491353.1
PDB 1K8W
1R3F
1ZL3
PDBsum 1K8W
1R3F
1ZL3
ProteinModelPortal P60340
SMR P60340
IntAct P60340
STRING 511145.b3166
PaxDb P60340
PRIDE P60340
EnsemblBacteria AAC76200
BAE77212
GeneID 12933440
947687
KEGG ecj:Y75_p3088
eco:b3166
PATRIC 32121750
EchoBASE EB1164
EcoGene EG11177
eggNOG COG0130
HOGENOM HOG000231223
InParanoid P60340
KO K03177
OMA YELQFIR
OrthoDB EOG6358D3
PhylomeDB P60340
BioCyc EcoCyc:EG11177-MONOMER
ECOL316407:JW3135-MONOMER
MetaCyc:EG11177-MONOMER
EvolutionaryTrace P60340
PRO PR:P60340
Proteomes UP000000318
UP000000625
Genevestigator P60340
GO GO:0009982
GO:0003723
GO:0001522
GO:0031119
Gene3D 2.30.130.10
HAMAP MF_01080
InterPro IPR002501
IPR020103
IPR015947
IPR014780
IPR015240
PANTHER PTHR13767
Pfam PF09157
PF01509
SUPFAM SSF55120
SSF88697
TIGRFAMs TIGR00431

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status

involved_in

GO:1990481

mRNA pseudouridine synthesis

PMID:21873635[1]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

PANTHER:PTN000349172
SGD:S000005236

P

Seeded From UniProt

complete

enables

GO:0009982

pseudouridine synthase activity

PMID:21873635[1]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

EcoGene:EG11177
PANTHER:PTN000349172
SGD:S000005236

F

Seeded From UniProt

complete

involved_in

GO:0006400

tRNA modification

PMID:21873635[1]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

PANTHER:PTN000349172
SGD:S000005236

P

Seeded From UniProt

complete

part_of

GO:0005739

mitochondrion

PMID:21873635[1]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

PANTHER:PTN000349172
SGD:S000005236

C

Seeded From UniProt

complete

involved_in

GO:0034337

RNA folding

PMID:27849601[2]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0031119

tRNA pseudouridine synthesis

PMID:11142385[3]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

enables

GO:0009982

pseudouridine synthase activity

PMID:7489483[4]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

part_of

GO:0005829

cytosol

PMID:18304323[5]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

involved_in

GO:0001522

pseudouridine synthesis

PMID:11142385[3]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

enables

GO:0000049

tRNA binding

PMID:27849601[2]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

involved_in

GO:0001522

pseudouridine synthesis

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR014780
InterPro:IPR015240
InterPro:IPR020103

P

Seeded From UniProt

complete

enables

GO:0003723

RNA binding

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR014780
InterPro:IPR015240
InterPro:IPR020103
InterPro:IPR036974

F

Seeded From UniProt

complete

involved_in

GO:0006396

RNA processing

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR002501

P

Seeded From UniProt

complete

involved_in

GO:0009451

RNA modification

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR014780
InterPro:IPR015240
InterPro:IPR020103

P

Seeded From UniProt

complete

enables

GO:0009982

pseudouridine synthase activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR014780
InterPro:IPR015240
InterPro:IPR020103

F

Seeded From UniProt

complete

enables

GO:0106029

tRNA pseudouridine synthase activity

GO_REF:0000003

ECO:0000501

evidence used in automatic assertion

EC:5.4.99.25

F

Seeded From UniProt

complete

enables

GO:0009982

pseudouridine synthase activity

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000100585

F

Seeded From UniProt

complete

involved_in

GO:0031119

tRNA pseudouridine synthesis

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000100585

P

Seeded From UniProt

complete

involved_in

GO:0008033

tRNA processing

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0819

P

Seeded From UniProt

complete

enables

GO:0016853

isomerase activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0413

F

Seeded From UniProt

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. 1.0 1.1 1.2 1.3 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page
  2. 2.0 2.1 Keffer-Wilkes, LC et al. (2016) RNA modification enzyme TruB is a tRNA chaperone. Proc. Natl. Acad. Sci. U.S.A. 113 14306-14311 PubMed GONUTS page
  3. 3.0 3.1 Gutgsell, N et al. (2000) Deletion of the Escherichia coli pseudouridine synthase gene truB blocks formation of pseudouridine 55 in tRNA in vivo, does not affect exponential growth, but confers a strong selective disadvantage in competition with wild-type cells. RNA 6 1870-81 PubMed GONUTS page
  4. Nurse, K et al. (1995) Purification, cloning, and properties of the tRNA psi 55 synthase from Escherichia coli. RNA 1 102-12 PubMed GONUTS page
  5. Ishihama, Y et al. (2008) Protein abundance profiling of the Escherichia coli cytosol. BMC Genomics 9 102 PubMed GONUTS page