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ECOLI:RPOE
Contents
| Species (Taxon ID) | Escherichia coli (strain K12). (83333) | |
| Gene Name(s) | rpoE (synonyms: sigE) | |
| Protein Name(s) | ECF RNA polymerase sigma-E factor
RNA polymerase sigma-E factor Sigma-24 | |
| External Links | ||
| UniProt | P0AGB6 | |
| EMBL | D13169 U37089 D64044 U00096 AP009048 U10148 | |
| PIR | I60227 | |
| RefSeq | NP_417068.1 YP_490801.1 | |
| PDB | 1OR7 2H27 | |
| PDBsum | 1OR7 2H27 | |
| ProteinModelPortal | P0AGB6 | |
| SMR | P0AGB6 | |
| DIP | DIP-10774N | |
| IntAct | P0AGB6 | |
| STRING | 511145.b2573 | |
| PaxDb | P0AGB6 | |
| PRIDE | P0AGB6 | |
| EnsemblBacteria | AAC75626 BAE76749 | |
| GeneID | 12934052 947050 | |
| KEGG | ecj:Y75_p2526 eco:b2573 | |
| PATRIC | 32120545 | |
| EchoBASE | EB1843 | |
| EcoGene | EG11897 | |
| eggNOG | COG1595 | |
| HOGENOM | HOG000094755 | |
| InParanoid | P0AGB6 | |
| KO | K03088 | |
| OMA | DAENYES | |
| OrthoDB | EOG6ZD6DW | |
| PhylomeDB | P0AGB6 | |
| BioCyc | EcoCyc:RPOE-MONOMER ECOL316407:JW2557-MONOMER MetaCyc:RPOE-MONOMER | |
| EvolutionaryTrace | P0AGB6 | |
| PRO | PR:P0AGB6 | |
| Proteomes | UP000000318 UP000000625 | |
| Genevestigator | P0AGB6 | |
| GO | GO:0005737 GO:0003677 GO:0003700 GO:0016987 GO:0006352 GO:0006355 GO:0006970 GO:0009266 | |
| Gene3D | 1.10.10.10 | |
| InterPro | IPR014284 IPR000838 IPR007627 IPR013249 IPR014286 IPR013325 IPR013324 IPR011991 | |
| Pfam | PF04542 PF08281 | |
| SUPFAM | SSF88659 SSF88946 | |
| TIGRFAMs | TIGR02939 TIGR02937 | |
| PROSITE | PS01063 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0016987 |
sigma factor activity |
ECO:0000315 |
F |
The authors created a plasmid construct with a promoterless Lacz and a random segment of E.coli Genomic DNA and transformed E.coli with it. RNA polymerase Sigma E factor fuctions under heat shock conditions. The colonies were placed under heat shock conditions. Those expressing Lacz were selected. These then went through a further selection process in order to determine the genes the sigma factor regualtes and the promoters it target. They also used mutants of RseA, which antagonized the sigma factor, to determine if RseA also regulates those genes effected by the sigma factor, as well as finding more promoters acted on by the sigma factor. |
complete | |||||
|
involved_in |
GO:0009266 |
response to temperature stimulus |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006970 |
response to osmotic stress |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006355 |
regulation of transcription, DNA-templated |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005737 |
cytoplasm |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:2000142 |
regulation of DNA-templated transcription, initiation |
ECO:0000366 |
evidence based on logical inference from automatic annotation used in automatic assertion |
GO:0016987 |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:2000142 |
regulation of DNA-templated transcription, initiation |
ECO:0000366 |
evidence based on logical inference from automatic annotation used in automatic assertion |
GO:0016987 |
P |
Seeded From UniProt |
complete | ||
|
enables |
GO:0003677 |
DNA binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003700 |
DNA-binding transcription factor activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
InterPro:IPR000838 |
F |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0006352 |
DNA-templated transcription, initiation |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
InterPro:IPR000838 |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0006355 |
regulation of transcription, DNA-templated |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
InterPro:IPR000838 |
P |
Seeded From UniProt |
complete | ||
|
enables |
GO:0016987 |
sigma factor activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006355 |
regulation of transcription, DNA-templated |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016987 |
sigma factor activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005737 |
cytoplasm |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003677 |
DNA binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ Dartigalongue, C et al. (2001) Characterization of the Escherichia coli sigma E regulon. J. Biol. Chem. 276 20866-75 PubMed GONUTS page
- ↑ Ades, SE et al. (2003) Regulation of the alternative sigma factor sigma(E) during initiation, adaptation, and shutoff of the extracytoplasmic heat shock response in Escherichia coli. J. Bacteriol. 185 2512-9 PubMed GONUTS page
- ↑ Bianchi, AA & Baneyx, F (1999) Hyperosmotic shock induces the sigma32 and sigmaE stress regulons of Escherichia coli. Mol. Microbiol. 34 1029-38 PubMed GONUTS page
- ↑ Danese, PN & Silhavy, TJ (1997) The sigma(E) and the Cpx signal transduction systems control the synthesis of periplasmic protein-folding enzymes in Escherichia coli. Genes Dev. 11 1183-93 PubMed GONUTS page
- ↑ Campbell, EA et al. (2003) Crystal structure of Escherichia coli sigmaE with the cytoplasmic domain of its anti-sigma RseA. Mol. Cell 11 1067-78 PubMed GONUTS page
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