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ECOLI:METH
Contents
| Species (Taxon ID) | Escherichia coli (strain K12). (83333) | |
| Gene Name(s) | metH | |
| Protein Name(s) | Methionine synthase
5-methyltetrahydrofolate--homocysteine methyltransferase Methionine synthase, vitamin-B12-dependent MS | |
| External Links | ||
| UniProt | P13009 | |
| EMBL | X16584 J04975 U00006 U00096 AP009048 | |
| PIR | B65209 | |
| RefSeq | NP_418443.1 YP_492162.1 | |
| PDB | 1BMT 1K7Y 1K98 1MSK 3BUL 3IV9 3IVA | |
| PDBsum | 1BMT 1K7Y 1K98 1MSK 3BUL 3IV9 3IVA | |
| ProteinModelPortal | P13009 | |
| SMR | P13009 | |
| IntAct | P13009 | |
| STRING | 511145.b4019 | |
| SWISS-2DPAGE | P13009 | |
| PaxDb | P13009 | |
| PRIDE | P13009 | |
| EnsemblBacteria | AAC76989 BAE78021 | |
| GeneID | 12934458 948522 | |
| KEGG | ecj:Y75_p3906 eco:b4019 | |
| PATRIC | 32123567 | |
| EchoBASE | EB0582 | |
| EcoGene | EG10587 | |
| eggNOG | COG1410 | |
| HOGENOM | HOG000251409 | |
| InParanoid | P13009 | |
| KO | K00548 | |
| OMA | DYNSIMV | |
| OrthoDB | EOG6091CH | |
| PhylomeDB | P13009 | |
| BioCyc | EcoCyc:HOMOCYSMETB12-MONOMER ECOL316407:JW3979-MONOMER MetaCyc:HOMOCYSMETB12-MONOMER RETL1328306-WGS:GSTH-2949-MONOMER | |
| UniPathway | UPA00051 | |
| EvolutionaryTrace | P13009 | |
| PRO | PR:P13009 | |
| Proteomes | UP000000318 UP000000625 | |
| Genevestigator | P13009 | |
| GO | GO:0005737 GO:0031419 GO:0008705 GO:0008898 GO:0008270 GO:0042558 | |
| Gene3D | 1.10.1240.10 3.10.196.10 3.20.20.20 3.20.20.330 3.40.50.280 | |
| InterPro | IPR003759 IPR006158 IPR011005 IPR011822 IPR000489 IPR003726 IPR004223 | |
| Pfam | PF02310 PF02607 PF02965 PF00809 PF02574 | |
| PIRSF | PIRSF000381 | |
| SMART | SM01018 | |
| SUPFAM | SSF47644 SSF51717 SSF52242 SSF56507 SSF82282 | |
| TIGRFAMs | TIGR02082 | |
| PROSITE | PS50974 PS51332 PS51337 PS50970 PS50972 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
|
enables |
GO:0008705 |
methionine synthase activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008705 |
methionine synthase activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0031419 |
cobalamin binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008276 |
protein methyltransferase activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0031419 |
cobalamin binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0050667 |
homocysteine metabolic process |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0035999 |
tetrahydrofolate interconversion |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0009086 |
methionine biosynthetic process |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008705 |
methionine synthase activity |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
EcoGene:EG10587 |
F |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005829 |
cytosol |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
EcoGene:EG10587 |
C |
Seeded From UniProt |
complete | ||
|
enables |
GO:0008705 |
methionine synthase activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005737 |
cytoplasm |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005737 |
cytoplasm |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0031419 |
cobalamin binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008705 |
methionine synthase activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008270 |
zinc ion binding |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005829 |
cytosol |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006479 |
protein methylation |
ECO:0000364 |
evidence based on logical inference from manual annotation used in automatic assertion |
GO:0008276 |
P |
Seeded From UniProt |
complete | ||
|
enables |
GO:0008270 |
zinc ion binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008705 |
methionine synthase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0009086 |
methionine biosynthetic process |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0031419 |
cobalamin binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
InterPro:IPR006158 |
F |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0042558 |
pteridine-containing compound metabolic process |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0044237 |
cellular metabolic process |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0046872 |
metal ion binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008705 |
methionine synthase activity |
ECO:0000501 |
evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0031419 |
cobalamin binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0009086 |
methionine biosynthetic process |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0032259 |
methylation |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008168 |
methyltransferase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0046872 |
metal ion binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0008652 |
cellular amino acid biosynthetic process |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016740 |
transferase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Drummond, JT et al. (1995) Characterization of nonradioactive assays for cobalamin-dependent and cobalamin-independent methionine synthase enzymes. Anal. Biochem. 228 323-9 PubMed GONUTS page
- ↑ 2.0 2.1 Grabowski, M et al. (2012) Role of heat-shock proteins and cobalamine in maintaining methionine synthase activity. Acta Biochim. Pol. 59 489-93 PubMed GONUTS page
- ↑ 3.0 3.1 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page
- ↑ Hall, DA et al. (2000) Interaction of flavodoxin with cobalamin-dependent methionine synthase. Biochemistry 39 10711-9 PubMed GONUTS page
- ↑ Jarrett, JT et al. (1997) Purification and assay of cobalamin-dependent methionine synthase from Escherichia coli. Meth. Enzymol. 281 196-213 PubMed GONUTS page
- ↑ Banerjee, RV et al. (1989) Cloning and sequence analysis of the Escherichia coli metH gene encoding cobalamin-dependent methionine synthase and isolation of a tryptic fragment containing the cobalamin-binding domain. J. Biol. Chem. 264 13888-95 PubMed GONUTS page
- ↑ Banerjee, RV et al. (1990) Participation of cob(I) alamin in the reaction catalyzed by methionine synthase from Escherichia coli: a steady-state and rapid reaction kinetic analysis. Biochemistry 29 11101-9 PubMed GONUTS page
- ↑ Goulding, CW et al. (1997) Cobalamin-dependent methionine synthase is a modular protein with distinct regions for binding homocysteine, methyltetrahydrofolate, cobalamin, and adenosylmethionine. Biochemistry 36 8082-91 PubMed GONUTS page
- ↑ Goulding, CW & Matthews, RG (1997) Cobalamin-dependent methionine synthase from Escherichia coli: involvement of zinc in homocysteine activation. Biochemistry 36 15749-57 PubMed GONUTS page
- ↑ Ishihama, Y et al. (2008) Protein abundance profiling of the Escherichia coli cytosol. BMC Genomics 9 102 PubMed GONUTS page
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