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ECOLI:LACY

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Species (Taxon ID) Escherichia coli (strain K12). (83333)
Gene Name(s) lacY
Protein Name(s) Lactose permease

Lactose-proton symport

External Links
UniProt P02920
EMBL J01636
V00295
X56095
U73857
U00096
AP009048
PIR A03418
RefSeq NP_414877.1
YP_488637.1
PDB 1M2U
1PV6
1PV7
2CFP
2CFQ
2V8N
2Y5Y
PDBsum 1M2U
1PV6
1PV7
2CFP
2CFQ
2V8N
2Y5Y
ProteinModelPortal P02920
SMR P02920
DIP DIP-10080N
STRING 511145.b0343
TCDB 2.A.1.5.1
PRIDE P02920
EnsemblBacteria AAC73446
BAE76125
GeneID 12934191
949083
KEGG ecj:Y75_p0332
eco:b0343
PATRIC 32115819
EchoBASE EB0521
EcoGene EG10526
eggNOG COG0477
HOGENOM HOG000114363
InParanoid P02920
KO K02532
OMA IVGCFKY
OrthoDB EOG6KHG2V
PhylomeDB P02920
BioCyc EcoCyc:LACY-MONOMER
ECOL316407:JW0334-MONOMER
MetaCyc:LACY-MONOMER
EvolutionaryTrace P02920
PRO PR:P02920
Proteomes UP000000318
UP000000625
Genevestigator P02920
GO GO:0016021
GO:0005887
GO:0016020
GO:0005886
GO:0030395
GO:0015528
GO:0005351
GO:0008643
GO:0015767
GO:0015992
InterPro IPR000576
IPR018457
IPR020846
Pfam PF01306
PRINTS PR00174
SUPFAM SSF103473
TIGRFAMs TIGR00882
PROSITE PS00896
PS00897
PS50850

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
GO:0015528

lactose:hydrogen symporter activity

PMID:3922794[1]

ECO:0000315

F

Table 1: Shows kinetic constants for mutant compared to wild-type for lactose uptake.

complete
CACAO 4140

GO:0008643

carbohydrate transport

PMID:22202176[2]

ECO:0000315

P

Figure 4 compares rates of IPTG uptake from the media between a lac Y mutant strain of E. coli and the wild type organism.

complete
CACAO 4177

GO:0016021

integral to membrane

PMID:6336750[3]

ECO:0000255

PMID:7108955[4]


C

Fig. 2

complete
CACAO 8706

enables

GO:0015528

lactose:proton symporter activity

PMID:3922794[1]

ECO:0000315

mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

involved_in

GO:0008643

carbohydrate transport

PMID:22202176[2]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

enables

GO:0030395

lactose binding

PMID:21873635[5]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

EcoGene:EG10526
PANTHER:PTN001922279

F

Seeded From UniProt

complete

enables

GO:0015528

lactose:proton symporter activity

PMID:21873635[5]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

EcoGene:EG10526
PANTHER:PTN001922279

F

Seeded From UniProt

complete

part_of

GO:0005887

integral component of plasma membrane

PMID:21873635[5]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

EcoGene:EG10526
EcoGene:EG10664
PANTHER:PTN001160664

C

Seeded From UniProt

complete

enables

GO:0030395

lactose binding

PMID:12893935[6]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

part_of

GO:0016021

integral component of membrane

PMID:12893935[6]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0016020

membrane

PMID:4900505[7]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

involved_in

GO:0008643

carbohydrate transport

PMID:5541536[8]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

enables

GO:0005351

carbohydrate:proton symporter activity

PMID:7000781[9]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

involved_in

GO:0015767

lactose transport

PMID:7028742[10]

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0015767

lactose transport

PMID:3520229[11]

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

enables

GO:0015528

lactose:proton symporter activity

PMID:7028742[10]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0015528

lactose:proton symporter activity

PMID:3520229[11]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

part_of

GO:0005887

integral component of plasma membrane

PMID:7028742[10]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0005886

plasma membrane

PMID:7028742[10]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0005886

plasma membrane

PMID:15919996[12]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

involved_in

GO:0034219

carbohydrate transmembrane transport

GO_REF:0000108

ECO:0000366

evidence based on logical inference from automatic annotation used in automatic assertion

GO:0005351

P

Seeded From UniProt

complete

involved_in

GO:0034219

carbohydrate transmembrane transport

GO_REF:0000108

ECO:0000364

evidence based on logical inference from manual annotation used in automatic assertion

GO:0005351

P

Seeded From UniProt

complete

involved_in

GO:0055085

transmembrane transport

GO_REF:0000108

ECO:0000366

evidence based on logical inference from automatic annotation used in automatic assertion

GO:0015293

P

Seeded From UniProt

complete

involved_in

GO:1902600

proton transmembrane transport

GO_REF:0000108

ECO:0000366

evidence based on logical inference from automatic annotation used in automatic assertion

GO:0005351

P

Seeded From UniProt

complete

involved_in

GO:1902600

proton transmembrane transport

GO_REF:0000108

ECO:0000364

evidence based on logical inference from manual annotation used in automatic assertion

GO:0005351

P

Seeded From UniProt

complete

enables

GO:0005351

carbohydrate:proton symporter activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR000576

F

Seeded From UniProt

complete

involved_in

GO:0008643

carbohydrate transport

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR000576

P

Seeded From UniProt

complete

part_of

GO:0016020

membrane

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR000576

C

Seeded From UniProt

complete

enables

GO:0015293

symporter activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0769

F

Seeded From UniProt

complete

part_of

GO:0005886

plasma membrane

GO_REF:0000037
GO_REF:0000037
GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-1003
UniProtKB-KW:KW-0997
UniProtKB-SubCell:SL-0037

C

Seeded From UniProt

complete

part_of

GO:0016021

integral component of membrane

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0812

C

Seeded From UniProt

complete

part_of

GO:0016020

membrane

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0472

C

Seeded From UniProt

complete

involved_in

GO:0008643

carbohydrate transport

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0762

P

Seeded From UniProt

complete

edit table

Notes

References

See Help:References for how to manage references in GONUTS.

  1. ↑ 1.0 1.1 Neuhaus, JM et al. (1985) Properties of a mutant lactose carrier of Escherichia coli with a Cys148----Ser148 substitution. FEBS Lett. 185 83-8 PubMed GONUTS page
  2. ↑ 2.0 2.1 Fernández-Castané, A et al. (2012) Evidencing the role of lactose permease in IPTG uptake by Escherichia coli in fed-batch high cell density cultures. J. Biotechnol. 157 391-8 PubMed GONUTS page
  3. ↑ Foster, DL et al. (1983) Structure of the lac carrier protein of Escherichia coli. J. Biol. Chem. 258 31-4 PubMed GONUTS page
  4. ↑ Kyte, J & Doolittle, RF (1982) A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157 105-32 PubMed GONUTS page
  5. ↑ 5.0 5.1 5.2 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page
  6. ↑ 6.0 6.1 Abramson, J et al. (2003) Structure and mechanism of the lactose permease of Escherichia coli. Science 301 610-5 PubMed GONUTS page
  7. ↑ Jones, TH & Kennedy, EP (1969) Characterization of the membrane protein component of the lactose transport system of Escherichia coli. J. Biol. Chem. 244 5981-7 PubMed GONUTS page
  8. ↑ Crandall, M & Koch, AL (1971) Temperature-sensitive mutants of Escherichia coli affecting beta-galactoside transport. J. Bacteriol. 105 609-19 PubMed GONUTS page
  9. ↑ Newman, MJ & Wilson, TH (1980) Solubilization and reconstitution of the lactose transport system from Escherichia coli. J. Biol. Chem. 255 10583-6 PubMed GONUTS page
  10. ↑ 10.0 10.1 10.2 10.3 Newman, MJ et al. (1981) Purification and reconstitution of functional lactose carrier from Escherichia coli. J. Biol. Chem. 256 11804-8 PubMed GONUTS page
  11. ↑ 11.0 11.1 Viitanen, P et al. (1986) Purification, reconstitution, and characterization of the lac permease of Escherichia coli. Meth. Enzymol. 125 429-52 PubMed GONUTS page
  12. ↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed GONUTS page
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