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ECOLI:LACI
Contents
| Species (Taxon ID) | Escherichia coli (strain K12). (83333) | |
| Gene Name(s) | lacI | |
| Protein Name(s) | Lactose operon repressor | |
| External Links | ||
| UniProt | P03023 | |
| EMBL | V00294 X58469 U86347 J01636 U72488 U78872 U78873 U78874 U73857 U00096 AP009048 | |
| PIR | A93198 | |
| RefSeq | NP_414879.3 YP_488639.1 | |
| PDB | 1CJG 1EFA 1JWL 1JYE 1JYF 1L1M 1LBG 1LBH 1LBI 1LCC 1LCD 1LQC 1LTP 1OSL 1TLF 1Z04 2BJC 2KEI 2KEJ 2KEK 2P9H 2PAF 2PE5 3EDC | |
| PDBsum | 1CJG 1EFA 1JWL 1JYE 1JYF 1L1M 1LBG 1LBH 1LBI 1LCC 1LCD 1LQC 1LTP 1OSL 1TLF 1Z04 2BJC 2KEI 2KEJ 2KEK 2P9H 2PAF 2PE5 3EDC | |
| DisProt | DP00433 | |
| ProteinModelPortal | P03023 | |
| SMR | P03023 | |
| BioGrid | 849401 | |
| DIP | DIP-10079N | |
| IntAct | P03023 | |
| MINT | MINT-6478062 | |
| STRING | 511145.b0345 | |
| PRIDE | P03023 | |
| EnsemblBacteria | AAC73448 BAE76127 | |
| GeneID | 12931674 945007 | |
| KEGG | ecj:Y75_p0334 eco:b0345 | |
| PATRIC | 32115823 | |
| EchoBASE | EB0520 | |
| EcoGene | EG10525 | |
| eggNOG | COG1609 | |
| HOGENOM | HOG000220179 | |
| InParanoid | P03023 | |
| OMA | DITISRY | |
| OrthoDB | EOG6SJJMM | |
| PhylomeDB | P03023 | |
| BioCyc | EcoCyc:PD00763 ECOL316407:JW0336-MONOMER | |
| EvolutionaryTrace | P03023 | |
| PRO | PR:P03023 | |
| Proteomes | UP000000318 UP000000625 | |
| Genevestigator | P03023 | |
| GO | GO:0005829 GO:0003677 GO:0003700 GO:0006351 | |
| Gene3D | 1.10.260.40 | |
| InterPro | IPR010982 IPR028082 IPR000843 | |
| Pfam | PF00356 | |
| PRINTS | PR00036 | |
| SMART | SM00354 | |
| SUPFAM | SSF47413 SSF53822 | |
| PROSITE | PS00356 PS50932 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0006355 |
regulation of transcription, DNA-dependent |
ECO:0000314 |
P |
Figure 5 shows that mutation at position 167 of LacI results in monomer-like profiles on a sucrose gradient instead of a functional tetramer as seen in wildtype LacI |
complete | |||||
| GO:0006355 |
regulation of transcription, DNA-dependent |
ECO:0000314 |
P |
Degradation of lacI mutant repressor fragments in vivo should be taken into account while assessing protein function of these mutants |
complete | |||||
|
part_of |
GO:0005829 |
cytosol |
ECO:0007005 |
high throughput direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0042802 |
identical protein binding |
ECO:0000353 |
physical interaction evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0042802 |
identical protein binding |
ECO:0000353 |
physical interaction evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0042802 |
identical protein binding |
ECO:0000353 |
physical interaction evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0045892 |
negative regulation of transcription, DNA-templated |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0001217 |
DNA-binding transcription repressor activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0000986 |
bacterial-type proximal promoter sequence-specific DNA binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003677 |
DNA binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006355 |
regulation of transcription, DNA-templated |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003677 |
DNA binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ Schmitz, A et al. (1976) Mutations affecting the quaternary structure of the lac repressor. J. Biol. Chem. 251 3359-66 PubMed GONUTS page
- ↑ Schlotmann, M & Beyreuther, K (1979) Degradation of the DNA-binding domain of wild-type and i-d lac repressors in Escherichia coli. Eur. J. Biochem. 95 39-49 PubMed GONUTS page
- ↑ 3.0 3.1 Lasserre, JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27 3306-21 PubMed GONUTS page
- ↑ Rutkauskas, D et al. (2009) Tetramer opening in LacI-mediated DNA looping. Proc. Natl. Acad. Sci. U.S.A. 106 16627-32 PubMed GONUTS page
- ↑ Bell, CE & Lewis, M (2000) A closer view of the conformation of the Lac repressor bound to operator. Nat. Struct. Biol. 7 209-14 PubMed GONUTS page
- ↑ Müller-Hill, B et al. (1968) Mutants that make more lac repressor. Proc. Natl. Acad. Sci. U.S.A. 59 1259-64 PubMed GONUTS page
- ↑ Lehming, N et al. (1987) The interaction of the recognition helix of lac repressor with lac operator. EMBO J. 6 3145-53 PubMed GONUTS page
- ↑ Riggs, AD et al. (1970) Lac repressor-operator interaction. I. Equilibrium studies. J. Mol. Biol. 48 67-83 PubMed GONUTS page
