ECOLI:IHFA

Species (Taxon ID)	Escherichia coli (strain K12). (83333)
Gene Name(s)	ihfA (synonyms: hid, himA)
Protein Name(s)	Integration host factor subunit alpha IHF-alpha
External Links
UniProt	P0A6X7
EMBL	K02844 V00291 U00096 AP009048 M14031
PIR	C23099
RefSeq	NP_416227.1 YP_489974.1
PDB	1IHF 1OUZ 1OWF 1OWG 2HT0 2IIE 2IIF
PDBsum	1IHF 1OUZ 1OWF 1OWG 2HT0 2IIE 2IIF
ProteinModelPortal	P0A6X7
SMR	P0A6X7
DIP	DIP-36031N
IntAct	P0A6X7
STRING	511145.b1712
PaxDb	P0A6X7
PRIDE	P0A6X7
EnsemblBacteria	AAC74782 BAA15480
GeneID	12931290 945472
KEGG	ecj:Y75_p1687 eco:b1712
PATRIC	32118730
EchoBASE	EB0435
EcoGene	EG10440
eggNOG	COG0776
HOGENOM	HOG000043829
InParanoid	P0A6X7
KO	K04764
OMA	FDLRDKK
OrthoDB	EOG615VS6
PhylomeDB	P0A6X7
BioCyc	EcoCyc:PD00347 ECOL316407:JW1702-MONOMER
EvolutionaryTrace	P0A6X7
PRO	PR:P0A6X7
Proteomes	UP000000318 UP000000625
Genevestigator	P0A6X7
GO	GO:0005737 GO:0003677 GO:0000746 GO:0006310 GO:0006355 GO:0006417 GO:0006351
Gene3D	4.10.520.10
HAMAP	MF_00380
InterPro	IPR000119 IPR020816 IPR010992 IPR005684
Pfam	PF00216
PRINTS	PR01727
SMART	SM00411
SUPFAM	SSF47729
TIGRFAMs	TIGR00987
PROSITE	PS00045

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Notes	Status
	GO:0032079	endodeoxyribonuclease activity	PMID:7499339^[1]	ECO:0000314			F	See Fig 3 & 5	complete
	GO:0032508	DNA duplex unwinding	PMID:19767439^[2]	ECO:0000314			P	See Fig 6	complete
NOT	GO:2000154	negative regulation of flagellar cell motility	PMID:12686630^[3]	ECO:0000315			P	Fig. 2. IHF does not mediate the repression of motility and flagellin production in E. coli strains W3110 and N99. (A) Immunoblot analysis. Proteins were extracted from wild-type and ihfA : : kan mutants of E. coli strains W3110 and N99 grown in DMEM to mid-exponential phase. The extracted proteins were subjected to SDS-PAGE followed by immunoblot analysis, using anti-flagellin antibodies. (B) Swarm plates were used to test the motility of W3110 and N99 wild-type and isogenic ihfA : : kan mutants. We compared E. coli K-12 W3110 and E. coli N99 with their isogenic ihfA mutants. In contrast to EPEC, the ihfA mutation of W3110 did not result in increased expression of fliC (Fig. 2A) or in increased motility, as seen under the microscope (data not shown) and confirmed by swarm plates (Fig. 2B).	complete
part_of	GO:0032993	protein-DNA complex	PMID:8419352^[4]	ECO:0005631	DNAse footprinting evidence used in manual assertion	RefSeq:NC_000913.2	C	Seeded From UniProt	complete
part_of	GO:0032993	protein-DNA complex	PMID:17293415^[5]	ECO:0005631	DNAse footprinting evidence used in manual assertion	RefSeq:NC_000913.2	C	Seeded From UniProt	complete
part_of	GO:0032993	protein-DNA complex	PMID:8419352^[4]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		C	Seeded From UniProt	complete
part_of	GO:0032993	protein-DNA complex	PMID:21778209^[6]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		C	Seeded From UniProt	complete
enables	GO:0001216	DNA-binding transcription activator activity	PMID:8419352^[4]	ECO:0005631	DNAse footprinting evidence used in manual assertion	RefSeq:NC_000913.2	F	Seeded From UniProt	complete
enables	GO:0001216	DNA-binding transcription activator activity	PMID:17293415^[5]	ECO:0005631	DNAse footprinting evidence used in manual assertion	RefSeq:NC_000913.2	F	Seeded From UniProt	complete
enables	GO:0001216	DNA-binding transcription activator activity	PMID:8419352^[4]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0001216	DNA-binding transcription activator activity	PMID:21778209^[6]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0000976	transcription regulatory region sequence-specific DNA binding	PMID:8419352^[4]	ECO:0005631	DNAse footprinting evidence used in manual assertion	RefSeq:NC_000913.2	F	Seeded From UniProt	complete
enables	GO:0000976	transcription regulatory region sequence-specific DNA binding	PMID:17293415^[5]	ECO:0005631	DNAse footprinting evidence used in manual assertion	RefSeq:NC_000913.2	F	Seeded From UniProt	complete
enables	GO:0000976	transcription regulatory region sequence-specific DNA binding	PMID:8419352^[4]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0000976	transcription regulatory region sequence-specific DNA binding	PMID:21778209^[6]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		F	Seeded From UniProt	complete
part_of	GO:0005829	cytosol	PMID:21873635^[7]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	EcoGene:EG10440 EcoGene:EG10441 EcoGene:EG10466 EcoGene:EG10467 PANTHER:PTN002110664	C	Seeded From UniProt	complete
enables	GO:0003677	DNA binding	PMID:21873635^[7]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	EcoGene:EG10466 EcoGene:EG10467 PANTHER:PTN002110658	F	Seeded From UniProt	complete
involved_in	GO:0006351	transcription, DNA-templated	PMID:8515442^[8]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0006351	transcription, DNA-templated	PMID:8230206^[9]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0006351	transcription, DNA-templated	PMID:7629095^[10]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0006351	transcription, DNA-templated	PMID:8515442^[8]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0006351	transcription, DNA-templated	PMID:8230206^[9]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0006351	transcription, DNA-templated	PMID:8156991^[11]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0006351	transcription, DNA-templated	PMID:7629095^[10]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0006351	transcription, DNA-templated	PMID:7862094^[12]	ECO:0000270	expression pattern evidence used in manual assertion		P	Seeded From UniProt	complete
part_of	GO:0005829	cytosol	PMID:18304323^[13]	ECO:0000314	direct assay evidence used in manual assertion		C	Seeded From UniProt	complete
involved_in	GO:0045893	positive regulation of transcription, DNA-templated	GO_REF:0000108	ECO:0000364	evidence based on logical inference from manual annotation used in automatic assertion	GO:0001216	P	Seeded From UniProt	complete
involved_in	GO:0045893	positive regulation of transcription, DNA-templated	GO_REF:0000108	ECO:0000364	evidence based on logical inference from manual annotation used in automatic assertion	GO:0001216	P	Seeded From UniProt	complete
involved_in	GO:0045893	positive regulation of transcription, DNA-templated	GO_REF:0000108	ECO:0000364	evidence based on logical inference from manual annotation used in automatic assertion	GO:0001216	P	Seeded From UniProt	complete
involved_in	GO:0045893	positive regulation of transcription, DNA-templated	GO_REF:0000108	ECO:0000364	evidence based on logical inference from manual annotation used in automatic assertion	GO:0001216	P	Seeded From UniProt	complete
enables	GO:0003677	DNA binding	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR000119 InterPro:IPR005684 InterPro:IPR010992 InterPro:IPR020816	F	Seeded From UniProt	complete
involved_in	GO:0006310	DNA recombination	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR005684	P	Seeded From UniProt	complete
involved_in	GO:0006355	regulation of transcription, DNA-templated	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR005684	P	Seeded From UniProt	complete
involved_in	GO:0006310	DNA recombination	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000001293	P	Seeded From UniProt	complete
involved_in	GO:0006355	regulation of transcription, DNA-templated	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000001293	P	Seeded From UniProt	complete
enables	GO:0003677	DNA binding	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000001293	F	Seeded From UniProt	complete
involved_in	GO:0006417	regulation of translation	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000001293	P	Seeded From UniProt	complete
enables	GO:0003677	DNA binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0238	F	Seeded From UniProt	complete
part_of	GO:0005737	cytoplasm	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0963	C	Seeded From UniProt	complete
involved_in	GO:0006310	DNA recombination	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0233	P	Seeded From UniProt	complete
involved_in	GO:0000746	conjugation	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0184	P	Seeded From UniProt	complete
involved_in	GO:0006417	regulation of translation	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0810	P	Seeded From UniProt	complete
part_of	GO:0009295	nucleoid	GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-SubCell:SL-0187	C	Seeded From UniProt	complete
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Notes

References

See Help:References for how to manage references in GONUTS.

↑ Nelson, WC et al. (1995) The traY gene product and integration host factor stimulate Escherichia coli DNA helicase I-catalyzed nicking at the F plasmid oriT. J. Biol. Chem. 270 28374-80 PubMed GONUTS page
↑ Sut, MV et al. (2009) Protein and DNA effectors control the TraI conjugative helicase of plasmid R1. J. Bacteriol. 191 6888-99 PubMed GONUTS page
↑ Yona-Nadler, C et al. (2003) Integration host factor (IHF) mediates repression of flagella in enteropathogenic and enterohaemorrhagic Escherichia coli. Microbiology (Reading, Engl.) 149 877-84 PubMed GONUTS page
↑ ^4.0 ^4.1 ^4.2 ^4.3 ^4.4 ^4.5 Schröder, I et al. (1993) Activation of the Escherichia coli nitrate reductase (narGHJI) operon by NarL and Fnr requires integration host factor. J. Biol. Chem. 268 771-4 PubMed GONUTS page
↑ ^5.0 ^5.1 ^5.2 Mettert, EL & Kiley, PJ (2007) Contributions of [4Fe-4S]-FNR and integration host factor to fnr transcriptional regulation. J. Bacteriol. 189 3036-43 PubMed GONUTS page
↑ ^6.0 ^6.1 ^6.2 Cagle, CA et al. (2011) Regulation of the integrase and cassette promoters of the class 1 integron by nucleoid-associated proteins. Microbiology (Reading, Engl.) 157 2841-53 PubMed GONUTS page
↑ ^7.0 ^7.1 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page
↑ ^8.0 ^8.1 Mengeritsky, G et al. (1993) Genetic and biochemical analysis of the integration host factor of Escherichia coli. J. Mol. Biol. 231 646-57 PubMed GONUTS page
↑ ^9.0 ^9.1 Granston, AE & Nash, HA (1993) Characterization of a set of integration host factor mutants deficient for DNA binding. J. Mol. Biol. 234 45-59 PubMed GONUTS page
↑ ^10.0 ^10.1 Zulianello, L et al. (1995) Participation of the flank regions of the integration host factor protein in the specificity and stability of DNA binding. J. Biol. Chem. 270 17902-7 PubMed GONUTS page
↑ Zulianello, L et al. (1994) The HimA and HimD subunits of integration host factor can specifically bind to DNA as homodimers. EMBO J. 13 1534-40 PubMed GONUTS page
↑ Presutti, DG & Hassan, HM (1995) Binding of integration host factor (IHF) to the Escherichia coli sodA gene and its role in the regulation of a sodA-lacZ fusion gene. Mol. Gen. Genet. 246 228-35 PubMed GONUTS page
↑ Ishihama, Y et al. (2008) Protein abundance profiling of the Escherichia coli cytosol. BMC Genomics 9 102 PubMed GONUTS page

[PMID:7499339-1] Nelson, WC et al. (1995) The traY gene product and integration host factor stimulate Escherichia coli DNA helicase I-catalyzed nicking at the F plasmid oriT. J. Biol. Chem. 270 28374-80 PubMed GONUTS page

[PMID:19767439-2] Sut, MV et al. (2009) Protein and DNA effectors control the TraI conjugative helicase of plasmid R1. J. Bacteriol. 191 6888-99 PubMed GONUTS page

[PMID:12686630-3] Yona-Nadler, C et al. (2003) Integration host factor (IHF) mediates repression of flagella in enteropathogenic and enterohaemorrhagic Escherichia coli. Microbiology (Reading, Engl.) 149 877-84 PubMed GONUTS page

[PMID:8419352-4] 4.0 ^4.1 ^4.2 ^4.3 ^4.4 ^4.5 Schröder, I et al. (1993) Activation of the Escherichia coli nitrate reductase (narGHJI) operon by NarL and Fnr requires integration host factor. J. Biol. Chem. 268 771-4 PubMed GONUTS page

[PMID:17293415-5] 5.0 ^5.1 ^5.2 Mettert, EL & Kiley, PJ (2007) Contributions of [4Fe-4S]-FNR and integration host factor to fnr transcriptional regulation. J. Bacteriol. 189 3036-43 PubMed GONUTS page

[PMID:21778209-6] 6.0 ^6.1 ^6.2 Cagle, CA et al. (2011) Regulation of the integrase and cassette promoters of the class 1 integron by nucleoid-associated proteins. Microbiology (Reading, Engl.) 157 2841-53 PubMed GONUTS page

[PMID:21873635-7] 7.0 ^7.1 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page

[PMID:8515442-8] 8.0 ^8.1 Mengeritsky, G et al. (1993) Genetic and biochemical analysis of the integration host factor of Escherichia coli. J. Mol. Biol. 231 646-57 PubMed GONUTS page

[PMID:8230206-9] 9.0 ^9.1 Granston, AE & Nash, HA (1993) Characterization of a set of integration host factor mutants deficient for DNA binding. J. Mol. Biol. 234 45-59 PubMed GONUTS page

[PMID:7629095-10] 10.0 ^10.1 Zulianello, L et al. (1995) Participation of the flank regions of the integration host factor protein in the specificity and stability of DNA binding. J. Biol. Chem. 270 17902-7 PubMed GONUTS page

[PMID:8156991-11] Zulianello, L et al. (1994) The HimA and HimD subunits of integration host factor can specifically bind to DNA as homodimers. EMBO J. 13 1534-40 PubMed GONUTS page

[PMID:7862094-12] Presutti, DG & Hassan, HM (1995) Binding of integration host factor (IHF) to the Escherichia coli sodA gene and its role in the regulation of a sodA-lacZ fusion gene. Mol. Gen. Genet. 246 228-35 PubMed GONUTS page

[PMID:18304323-13] Ishihama, Y et al. (2008) Protein abundance profiling of the Escherichia coli cytosol. BMC Genomics 9 102 PubMed GONUTS page

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ECOLI:IHFA

Contents

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