ECOLI:FIS

Species (Taxon ID)	Escherichia coli (strain K12). (83333)
Gene Name(s)	fis (ECO:0000255 with HAMAP-Rule:MF_00166)
Protein Name(s)	DNA-binding protein Fis (ECO:0000255 with HAMAP-Rule:MF_00166) Factor-for-inversion stimulation protein Hin recombinational enhancer-binding protein
External Links
UniProt	P0A6R3
EMBL	J03245 J03816 M95784 U18997 U00096 AP009048
PIR	A32142
RefSeq	NP_417727.1 YP_491443.1
PDB	1ETK 1ETO 1ETQ 1ETV 1ETW 1ETX 1ETY 1F36 1FIA 1FIP 3FIS 3IV5 3JR9 3JRA 3JRB 3JRC 3JRD 3JRE 3JRF 3JRG 3JRH 3JRI 4FIS
PDBsum	1ETK 1ETO 1ETQ 1ETV 1ETW 1ETX 1ETY 1F36 1FIA 1FIP 3FIS 3IV5 3JR9 3JRA 3JRB 3JRC 3JRD 3JRE 3JRF 3JRG 3JRH 3JRI 4FIS
DisProt	DP00422
ProteinModelPortal	P0A6R3
SMR	P0A6R3
DIP	DIP-47975N
IntAct	P0A6R3
MINT	MINT-1225073
STRING	511145.b3261
PaxDb	P0A6R3
PRIDE	P0A6R3
EnsemblBacteria	AAC76293 BAE77302
GeneID	12932870 947697
KEGG	ecj:Y75_p3179 eco:b3261
PATRIC	32121952
EchoBASE	EB0313
EcoGene	EG10317
eggNOG	COG2901
HOGENOM	HOG000256331
InParanoid	P0A6R3
KO	K03557
OMA	MSDNANL
OrthoDB	EOG67HJXZ
PhylomeDB	P0A6R3
BioCyc	EcoCyc:PD00196 ECOL316407:JW3229-MONOMER
EvolutionaryTrace	P0A6R3
PRO	PR:P0A6R3
Proteomes	UP000000318 UP000000625
Genevestigator	P0A6R3
GO	GO:0000787 GO:0003677 GO:0043565 GO:0003700 GO:0034401 GO:0006355 GO:0006351
Gene3D	1.10.10.60
HAMAP	MF_00166
InterPro	IPR005412 IPR009057 IPR002197
Pfam	PF02954
PIRSF	PIRSF002097
PRINTS	PR01591 PR01590
SUPFAM	SSF46689

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Notes	Status
	GO:0003677	DNA binding	PMID:21097887^[1]	ECO:0000314			F	Figure 4 demonstrates temporal patterns of Fis binding to the bacterial chromosome.	complete
involved_in	GO:0045911	positive regulation of DNA recombination	PMID:9362499^[2]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
enables	GO:0000985	bacterial-type RNA polymerase core promoter sequence-specific DNA binding	PMID:9362499^[2]	ECO:0000315	mutant phenotype evidence used in manual assertion		F	Seeded From UniProt	complete
part_of	GO:0031421	invertasome	PMID:9362499^[2]	ECO:0000315	mutant phenotype evidence used in manual assertion		C	Seeded From UniProt	complete
enables	GO:0042803	protein homodimerization activity	PMID:1946369^[3]	ECO:0000315	mutant phenotype evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0042803	protein homodimerization activity	PMID:1619650^[4]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0042803	protein homodimerization activity	PMID:9362499^[2]	ECO:0000315	mutant phenotype evidence used in manual assertion		F	Seeded From UniProt	complete
involved_in	GO:0032359	provirus excision	PMID:9362499^[2]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
enables	GO:0044374	sequence-specific DNA binding, bending	PMID:9362499^[2]	ECO:0000315	mutant phenotype evidence used in manual assertion		F	Seeded From UniProt	complete
involved_in	GO:0001121	bacterial transcription	PMID:9362499^[2]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
enables	GO:0001216	DNA-binding transcription activator activity	PMID:9362499^[2]	ECO:0000315	mutant phenotype evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0042803	protein homodimerization activity	PMID:11183780^[5]	ECO:0000315	mutant phenotype evidence used in manual assertion		F	Seeded From UniProt	complete
part_of	GO:0032993	protein-DNA complex	PMID:21778209^[6]	ECO:0005631	DNAse footprinting evidence used in manual assertion	RefSeq:NC_019062.1	C	Seeded From UniProt	complete
part_of	GO:0032993	protein-DNA complex	PMID:21778209^[6]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		C	Seeded From UniProt	complete
enables	GO:0001217	DNA-binding transcription repressor activity	PMID:21778209^[6]	ECO:0005631	DNAse footprinting evidence used in manual assertion	RefSeq:NC_019062.1	F	Seeded From UniProt	complete
enables	GO:0001217	DNA-binding transcription repressor activity	PMID:21778209^[6]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0000976	transcription regulatory region sequence-specific DNA binding	PMID:21778209^[6]	ECO:0005631	DNAse footprinting evidence used in manual assertion	RefSeq:NC_019062.1	F	Seeded From UniProt	complete
enables	GO:0000976	transcription regulatory region sequence-specific DNA binding	PMID:21778209^[6]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0043565	sequence-specific DNA binding	PMID:21873635^[7]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	EcoGene:EG10317 PANTHER:PTN002936601	F	Seeded From UniProt	complete
involved_in	GO:0034401	chromatin organization involved in regulation of transcription	PMID:21873635^[7]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	EcoGene:EG10317 PANTHER:PTN002936601	P	Seeded From UniProt	complete
involved_in	GO:0006355	regulation of transcription, DNA-templated	PMID:21873635^[7]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	EcoGene:EG10317 PANTHER:PTN002936601	P	Seeded From UniProt	complete
part_of	GO:0000787	cytoplasmic nucleosome	PMID:21873635^[7]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	EcoGene:EG10317 PANTHER:PTN002936601	C	Seeded From UniProt	complete
enables	GO:0043565	sequence-specific DNA binding	PMID:10551881^[8]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
involved_in	GO:0034401	chromatin organization involved in regulation of transcription	PMID:11812843^[9]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0009314	response to radiation	PMID:27718375^[10]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0006355	regulation of transcription, DNA-templated	PMID:17233826^[11]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
part_of	GO:0005829	cytosol	PMID:18304323^[12]	ECO:0000314	direct assay evidence used in manual assertion		C	Seeded From UniProt	complete
part_of	GO:0005829	cytosol	PMID:15911532^[13]	ECO:0000314	direct assay evidence used in manual assertion		C	Seeded From UniProt	complete
enables	GO:0003677	DNA binding	PMID:18340041^[14]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
part_of	GO:0000787	cytoplasmic nucleosome	PMID:10320479^[15]	ECO:0000314	direct assay evidence used in manual assertion		C	Seeded From UniProt	complete
involved_in	GO:0045892	negative regulation of transcription, DNA-templated	GO_REF:0000108	ECO:0000364	evidence based on logical inference from manual annotation used in automatic assertion	GO:0001217	P	Seeded From UniProt	complete
involved_in	GO:0045892	negative regulation of transcription, DNA-templated	GO_REF:0000108	ECO:0000364	evidence based on logical inference from manual annotation used in automatic assertion	GO:0001217	P	Seeded From UniProt	complete
involved_in	GO:0045893	positive regulation of transcription, DNA-templated	GO_REF:0000108	ECO:0000364	evidence based on logical inference from manual annotation used in automatic assertion	GO:0001216	P	Seeded From UniProt	complete
enables	GO:0003677	DNA binding	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR005412 InterPro:IPR009057	F	Seeded From UniProt	complete
involved_in	GO:0006355	regulation of transcription, DNA-templated	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR005412	P	Seeded From UniProt	complete
enables	GO:0043565	sequence-specific DNA binding	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR002197	F	Seeded From UniProt	complete
enables	GO:0003700	DNA-binding transcription factor activity	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000079176	F	Seeded From UniProt	complete
involved_in	GO:0006355	regulation of transcription, DNA-templated	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000079176	P	Seeded From UniProt	complete
part_of	GO:0005737	cytoplasm	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0963	C	Seeded From UniProt	complete
enables	GO:0003677	DNA binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0238	F	Seeded From UniProt	complete
part_of	GO:0009295	nucleoid	GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-SubCell:SL-0187	C	Seeded From UniProt	complete
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Notes

References

See Help:References for how to manage references in GONUTS.

↑ Kahramanoglou, C et al. (2011) Direct and indirect effects of H-NS and Fis on global gene expression control in Escherichia coli. Nucleic Acids Res. 39 2073-91 PubMed GONUTS page
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 Safo, MK et al. (1997) The transactivation region of the fis protein that controls site-specific DNA inversion contains extended mobile beta-hairpin arms. EMBO J. 16 6860-73 PubMed GONUTS page
↑ Yuan, HS et al. (1991) The molecular structure of wild-type and a mutant Fis protein: relationship between mutational changes and recombinational enhancer function or DNA binding. Proc. Natl. Acad. Sci. U.S.A. 88 9558-62 PubMed GONUTS page
↑ Kostrewa, D et al. (1992) Crystal structure of the factor for inversion stimulation FIS at 2.0 A resolution. J. Mol. Biol. 226 209-26 PubMed GONUTS page
↑ Cheng, YS et al. (2000) Structural analysis of the transcriptional activation region on Fis: crystal structures of six Fis mutants with different activation properties. J. Mol. Biol. 302 1139-51 PubMed GONUTS page
↑ ^6.0 ^6.1 ^6.2 ^6.3 ^6.4 ^6.5 Cagle, CA et al. (2011) Regulation of the integrase and cassette promoters of the class 1 integron by nucleoid-associated proteins. Microbiology (Reading, Engl.) 157 2841-53 PubMed GONUTS page
↑ ^7.0 ^7.1 ^7.2 ^7.3 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page
↑ Azam, TA & Ishihama, A (1999) Twelve species of the nucleoid-associated protein from Escherichia coli. Sequence recognition specificity and DNA binding affinity. J. Biol. Chem. 274 33105-13 PubMed GONUTS page
↑ Schneider, R et al. (2001) An architectural role of the Escherichia coli chromatin protein FIS in organising DNA. Nucleic Acids Res. 29 5107-14 PubMed GONUTS page
↑ Sargentini, NJ et al. () Screen for genes involved in radiation survival of Escherichia coli and construction of a reference database. Mutat. Res. 793-794 1-14 PubMed GONUTS page
↑ Weinstein-Fischer, D & Altuvia, S (2007) Differential regulation of Escherichia coli topoisomerase I by Fis. Mol. Microbiol. 63 1131-44 PubMed GONUTS page
↑ Ishihama, Y et al. (2008) Protein abundance profiling of the Escherichia coli cytosol. BMC Genomics 9 102 PubMed GONUTS page
↑ Lopez-Campistrous, A et al. (2005) Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth. Mol. Cell Proteomics 4 1205-9 PubMed GONUTS page
↑ Cho, BK et al. (2008) Genome-wide analysis of Fis binding in Escherichia coli indicates a causative role for A-/AT-tracts. Genome Res. 18 900-10 PubMed GONUTS page
↑ Ishihama, A (1999) Modulation of the nucleoid, the transcription apparatus, and the translation machinery in bacteria for stationary phase survival. Genes Cells 4 135-43 PubMed GONUTS page

[PMID:21097887-1] Kahramanoglou, C et al. (2011) Direct and indirect effects of H-NS and Fis on global gene expression control in Escherichia coli. Nucleic Acids Res. 39 2073-91 PubMed GONUTS page

[PMID:9362499-2] 2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 Safo, MK et al. (1997) The transactivation region of the fis protein that controls site-specific DNA inversion contains extended mobile beta-hairpin arms. EMBO J. 16 6860-73 PubMed GONUTS page

[PMID:1946369-3] Yuan, HS et al. (1991) The molecular structure of wild-type and a mutant Fis protein: relationship between mutational changes and recombinational enhancer function or DNA binding. Proc. Natl. Acad. Sci. U.S.A. 88 9558-62 PubMed GONUTS page

[PMID:1619650-4] Kostrewa, D et al. (1992) Crystal structure of the factor for inversion stimulation FIS at 2.0 A resolution. J. Mol. Biol. 226 209-26 PubMed GONUTS page

[PMID:11183780-5] Cheng, YS et al. (2000) Structural analysis of the transcriptional activation region on Fis: crystal structures of six Fis mutants with different activation properties. J. Mol. Biol. 302 1139-51 PubMed GONUTS page

[PMID:21778209-6] 6.0 ^6.1 ^6.2 ^6.3 ^6.4 ^6.5 Cagle, CA et al. (2011) Regulation of the integrase and cassette promoters of the class 1 integron by nucleoid-associated proteins. Microbiology (Reading, Engl.) 157 2841-53 PubMed GONUTS page

[PMID:21873635-7] 7.0 ^7.1 ^7.2 ^7.3 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page

[PMID:10551881-8] Azam, TA & Ishihama, A (1999) Twelve species of the nucleoid-associated protein from Escherichia coli. Sequence recognition specificity and DNA binding affinity. J. Biol. Chem. 274 33105-13 PubMed GONUTS page

[PMID:11812843-9] Schneider, R et al. (2001) An architectural role of the Escherichia coli chromatin protein FIS in organising DNA. Nucleic Acids Res. 29 5107-14 PubMed GONUTS page

[PMID:27718375-10] Sargentini, NJ et al. () Screen for genes involved in radiation survival of Escherichia coli and construction of a reference database. Mutat. Res. 793-794 1-14 PubMed GONUTS page

[PMID:17233826-11] Weinstein-Fischer, D & Altuvia, S (2007) Differential regulation of Escherichia coli topoisomerase I by Fis. Mol. Microbiol. 63 1131-44 PubMed GONUTS page

[PMID:18304323-12] Ishihama, Y et al. (2008) Protein abundance profiling of the Escherichia coli cytosol. BMC Genomics 9 102 PubMed GONUTS page

[PMID:15911532-13] Lopez-Campistrous, A et al. (2005) Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth. Mol. Cell Proteomics 4 1205-9 PubMed GONUTS page

[PMID:18340041-14] Cho, BK et al. (2008) Genome-wide analysis of Fis binding in Escherichia coli indicates a causative role for A-/AT-tracts. Genome Res. 18 900-10 PubMed GONUTS page

[PMID:10320479-15] Ishihama, A (1999) Modulation of the nucleoid, the transcription apparatus, and the translation machinery in bacteria for stationary phase survival. Genes Cells 4 135-43 PubMed GONUTS page

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ECOLI:FIS

Contents

Annotations

Notes

References

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