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ECOLI:AMY1

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Species (Taxon ID) Escherichia coli (strain K12). (83333)
Gene Name(s) malS
Protein Name(s) Alpha-amylase

1,4-alpha-D-glucan glucanohydrolase

External Links
UniProt P25718
EMBL X58994
U00039
U00096
AP009048
PIR S23807
RefSeq NP_418028.1
YP_491863.1
ProteinModelPortal P25718
SMR P25718
DIP DIP-10148N
IntAct P25718
MINT MINT-1293484
STRING 511145.b3571
CAZy GH13
PRIDE P25718
EnsemblBacteria AAC76595
BAE77722
GeneID 12932718
948088
KEGG ecj:Y75_p3604
eco:b3571
PATRIC 32122618
EchoBASE EB1292
EcoGene EG11316
eggNOG COG0366
HOGENOM HOG000273912
InParanoid P25718
KO K01176
OMA FNWHNAT
OrthoDB EOG65QWH3
PhylomeDB P25718
BioCyc EcoCyc:ALPHA-AMYL-PERI-MONOMER
ECOL316407:JW3543-MONOMER
MetaCyc:ALPHA-AMYL-PERI-MONOMER
PRO PR:P25718
Proteomes UP000000318
UP000000625
Genevestigator P25718
GO GO:0030288
GO:0042597
GO:0004556
GO:0005509
GO:0030980
GO:0051692
Gene3D 3.20.20.80
InterPro IPR014635
IPR015902
IPR006047
IPR006589
IPR013781
IPR017853
PANTHER PTHR10357
Pfam PF00128
PIRSF PIRSF036917
SMART SM00642
SUPFAM SSF51445

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status

involved_in

GO:0051692

cellular oligosaccharide catabolic process

PMID:3005273[1]

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

part_of

GO:0042597

periplasmic space

PMID:3005273[1]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

enables

GO:0004556

alpha-amylase activity

PMID:3005273[1]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

involved_in

GO:0030980

alpha-glucan catabolic process

PMID:3005273[1]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

part_of

GO:0030288

outer membrane-bounded periplasmic space

PMID:3005273[1]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0030288

outer membrane-bounded periplasmic space

PMID:24140104[2]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

enables

GO:0005509

calcium ion binding

PMID:9268356[3]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0004556

alpha-amylase activity

PMID:9268356[3]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0003824

catalytic activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR006047

F

Seeded From UniProt

complete

enables

GO:0004556

alpha-amylase activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR014635

F

Seeded From UniProt

complete

enables

GO:0005509

calcium ion binding

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR014635

F

Seeded From UniProt

complete

involved_in

GO:0005975

carbohydrate metabolic process

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR006047

P

Seeded From UniProt

complete

involved_in

GO:0030980

alpha-glucan catabolic process

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR014635

P

Seeded From UniProt

complete

part_of

GO:0042597

periplasmic space

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR014635

C

Seeded From UniProt

complete

involved_in

GO:0051692

cellular oligosaccharide catabolic process

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR014635

P

Seeded From UniProt

complete

enables

GO:0004556

alpha-amylase activity

GO_REF:0000003

ECO:0000501

evidence used in automatic assertion

EC:3.2.1.1

F

Seeded From UniProt

complete

enables

GO:0103025

alpha-amylase activity (releasing maltohexaose)

GO_REF:0000003

ECO:0000501

evidence used in automatic assertion

EC:3.2.1.1

F

Seeded From UniProt

complete

involved_in

GO:0005975

carbohydrate metabolic process

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0119

P

Seeded From UniProt

complete

enables

GO:0016798

hydrolase activity, acting on glycosyl bonds

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0326

F

Seeded From UniProt

complete

enables

GO:0046872

metal ion binding

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0479

F

Seeded From UniProt

complete

part_of

GO:0042597

periplasmic space

GO_REF:0000037
GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0574
UniProtKB-SubCell:SL-0200

C

Seeded From UniProt

complete

involved_in

GO:0008152

metabolic process

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0326

P

Seeded From UniProt

complete

enables

GO:0016787

hydrolase activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0378

F

Seeded From UniProt

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. 1.0 1.1 1.2 1.3 1.4 Freundlieb, S & Boos, W (1986) Alpha-amylase of Escherichia coli, mapping and cloning of the structural gene, malS, and identification of its product as a periplasmic protein. J. Biol. Chem. 261 2946-53 PubMed GONUTS page
  2. Han, MJ et al. (2014) Comparison of the large-scale periplasmic proteomes of the Escherichia coli K-12 and B strains. J. Biosci. Bioeng. 117 437-42 PubMed GONUTS page
  3. 3.0 3.1 Spiess, C et al. (1997) Biochemical characterization and mass spectrometric disulfide bond mapping of periplasmic alpha-amylase MalS of Escherichia coli. J. Biol. Chem. 272 22125-33 PubMed GONUTS page