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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesTRIRU:LAP2Jwohlers, alpha12011-11-30 15:07:50 CSTGO:0004177 aminopeptidase activity (F)PMID:15632434ECO:0000269 experimental evidence used in manual assertion

Figures 1 and 2: Western Blot, Infer aminopeptidase activity through interaction.

Tables 6 and 7 and Figures 3 and 4: Sequence similarity/ alignment and activity of various Lap2 genes is comparable.

acceptableRHORU:NADBJwohlers, alpha12011-11-30 14:56:21 CSTGO:0009435 NAD biosynthetic process (P)PMID:7721706ECO:0000315 mutant phenotype evidence used in manual assertion

"In the course of this study, we also identified the R. rubrum nadBC genes. This conclusion is based on their very strong sequence similarity to nadBC in E. coli (18, 20) and on the Nic2 phenotype caused by their deletion from the R. rubrum chromosome. In contrast to the homologous genes of E. coli, which map to different regions of the chromosome (19), the nadB and nadC genes of R. rubrum appear to form an operon.

In R. rubrum, the juxtaposition of the coo and nad genes appears to be coincidental, as no physiological connection between the two has been established: the nad deletion confers a Nic2 phenotype in the absence of coo expression, and the insertion in cooA causes no apparent Nic 2 phenotype in a nad1 background."

acceptableRHORU:COOFJwohlers, alpha12011-11-30 14:28:13 CSTGO:0019410 aerobic respiration, using carbon monoxide as electron donor (P)PMID:1644755ECO:0000315 mutant phenotype evidence used in manual assertion

Table 2. Insertion into gene prevents H2 formation from CO.


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