Category:Team Holliday

Status	Page	User	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
requireschanges	RHIRD:G8XNW6	Bmmcdowell, Team Holliday	2015-03-26 12:40:06 CDT	GO:0030613 oxidoreductase activity, acting on phosphorus or arsenic in donors (F)	PMID:22176720	ECO:0000315 mutant phenotype evidence used in manual assertion	In figure 1 the AsIII oxidation profiles of wild type and mutant delta aioX and M53 mutants suggest that AioX participates to AsIII oxidation in Agrobacterium tumefaciens.	challenge
unacceptable	RHIRD:G8XNW6	Bmmcdowell, Team Holliday	2015-03-26 12:11:01 CDT	GO:0046872 metal ion binding (F)	PMID:22176720	ECO:0000314 direct assay evidence used in manual assertion	Gel filtration chromatography data suggests that AioX interacts with AsIII (figure 4). In figure 5A ICP-MS analysis directly demonstrates AsIII binding to AioX. Figure 5B fluorescence quenching data suggests the formation of an AioX–AsIII complex.	challenge
requireschanges	RHIRD:G8XNW6	Bmmcdowell, Team Holliday	2015-03-26 11:52:04 CDT	GO:0098562 cytoplasmic side of membrane (C)	PMID:22176720	ECO:0000314 direct assay evidence used in manual assertion	In Figure 2 a western blot analysis shows that AioX is associated with the cytoplasmic membrane.	challenge
flagged	RHIRD:H0H8Z6	Bmmcdowell, Team Holliday	2015-03-25 14:16:10 CDT	GO:0016987 sigma factor activity (F)	PMID:22660703	ECO:0000315 mutant phenotype evidence used in manual assertion	RpoN is a sigma factor for the aioBA genes. Figure 3 shows the effect of RpoN on aioBA expression.	challenge

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