CLOAB:Q9ANR5

Species (Taxon ID)	Clostridium acetobutylicum. (1488)
Gene Name(s)	adhE2 (ECO:0000313 with EMBL:AAK09379.1)
Protein Name(s)	Aldehyde-alcohol dehydrogenase (ECO:0000256 with PIRNR:PIRNR000111)
External Links
UniProt	Q9ANR5
EMBL	AF321779
RefSeq	NP_149199.1 YP_009076789.1
ProteinModelPortal	Q9ANR5
STRING	272562.CA_P0035
GeneID	1116040
KEGG	cac:CA_P0035
PATRIC	32033950
HOGENOM	HOG000025256
KO	K04072
OrthoDB	EOG6TFCQS
BioCyc	MetaCyc:MONOMER-15847
GO	GO:0008774 GO:0004022 GO:0004029 GO:0046872 GO:0071271 GO:0015976
Gene3D	3.40.309.10 3.40.605.10
InterPro	IPR001670 IPR018211 IPR016161 IPR016163 IPR016162 IPR015590 IPR012079
Pfam	PF00171 PF00465
PIRSF	PIRSF000111
SUPFAM	SSF53720
PROSITE	PS00913 PS00060

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
involved_in	GO:0071271	1-butanol biosynthetic process	PMID:11790753	ECO:0000314	direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
enables	GO:0004029	aldehyde dehydrogenase (NAD) activity	PMID:11790753	ECO:0000314	direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
enables	GO:0004022	alcohol dehydrogenase (NAD) activity	PMID:11790753	ECO:0000314	direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
enables	GO:0004022	alcohol dehydrogenase (NAD) activity	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR012079 InterPro:IPR034789	F		Seeded From UniProt	complete
involved_in	GO:0006066	alcohol metabolic process	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR012079	P		Seeded From UniProt	complete
enables	GO:0008774	acetaldehyde dehydrogenase (acetylating) activity	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR012079	F		Seeded From UniProt	complete
involved_in	GO:0015976	carbon utilization	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR012079	P		Seeded From UniProt	complete
enables	GO:0016491	oxidoreductase activity	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR001670 InterPro:IPR015590 InterPro:IPR016161 InterPro:IPR016162 InterPro:IPR016163 InterPro:IPR018211	F		Seeded From UniProt	complete
enables	GO:0016620	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR016163	F		Seeded From UniProt	complete
	GO:0004022	alcohol dehydrogenase (NAD) activity	PMID:11790753	ECO:0000314			F		See figures 1, 2, 5. The adhE2 gene of Clostridium acetobutylicum ATCC 824, coding for an aldehyde/alcohol dehydrogenase (AADH), was characterized from molecular and biochemical points of view. The 2,577-bp adhE2 codes for a 94.4-kDa protein. adhE2 is expressed, as a monocistronic operon, in alcohologenic cultures and not in solventogenic cultures. Primer extension analysis identified two transcriptional start sites 160 and 215 bp upstream of the adhE2 start codon. The expression of adhE2 from a plasmid in the DG1 mutant of C. acetobutylicum, a mutant cured of the pSOL1 megaplasmid, restored butanol production and provided elevated activities of NADH-dependent butyraldehyde and butanol dehydrogenases. The recombinant AdhE2 protein expressed in E. coli as a Strep-tag fusion protein and purified to homogeneity also demonstrated NADH-dependent butyraldehyde and butanol dehydrogenase activities. This is the second AADH identified in C. acetobutylicum ATCC 824, and to our knowledge this is the first example of a bacterium with two AADHs. It is noteworthy that the two corresponding genes, adhE and adhE2, are carried by the pSOL1 megaplasmid of C. acetobutylicum ATCC 824.	complete CACAO 3323
enables	GO:0046872	metal ion binding	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR001670 InterPro:IPR018211	F		Seeded From UniProt	complete
involved_in	GO:0055114	oxidation-reduction process	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR001670 InterPro:IPR012079 InterPro:IPR015590 InterPro:IPR016161 InterPro:IPR016162 InterPro:IPR016163 InterPro:IPR018211	P		Seeded From UniProt	complete
enables	GO:0016491	oxidoreductase activity	GO_REF:0000038	ECO:0000323	imported automatically asserted information used in automatic assertion	UniProtKB-KW:KW-0560	F		Seeded From UniProt	complete
involved_in	GO:0055114	oxidation-reduction process	GO_REF:0000038	ECO:0000323	imported automatically asserted information used in automatic assertion	UniProtKB-KW:KW-0560	P		Seeded From UniProt	complete
enables	GO:0004022	alcohol dehydrogenase (NAD) activity	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000132767	F		Seeded From UniProt	complete
enables	GO:0008774	acetaldehyde dehydrogenase (acetylating) activity	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000132767	F		Seeded From UniProt	complete
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References

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