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BPT4:UVSW
Contents
| Species (Taxon ID) | Enterobacteria phage T4 (Bacteriophage T4). (10665) | |
| Gene Name(s) | uvsW (synonyms: dar) | |
| Protein Name(s) | ATP-dependent DNA helicase uvsW
Dar protein | |
| External Links | ||
| UniProt | P20703 | |
| EMBL | AF158101 X56097 X14869 | |
| PIR | PQ0097 | |
| RefSeq | NP_049796.1 | |
| PDB | 1RIF 2JPN 2OCA | |
| PDBsum | 1RIF 2JPN 2OCA | |
| ProteinModelPortal | P20703 | |
| SMR | P20703 | |
| GeneID | 1258733 | |
| KEGG | vg:1258733 | |
| KO | K18959 | |
| EvolutionaryTrace | P20703 | |
| Proteomes | UP000009087 | |
| GO | GO:0005524 GO:0003677 GO:0004386 | |
| Gene3D | 3.40.50.300 | |
| InterPro | IPR020975 IPR006935 IPR014001 IPR001650 IPR027417 | |
| Pfam | PF00271 PF04851 PF11637 | |
| SMART | SM00487 SM00490 | |
| SUPFAM | SSF52540 | |
| PROSITE | PS51192 PS51194 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0008026 |
ATP-dependent helicase activity |
ECO:0000314 |
F |
In figure 6, y-branched linear dsDNA is incubated with a GST/uvsW fusion protein and single-stranded products are observed, indicating the unwinding (i.e., helicase) activity of the protein. In lane 9 of the gel, removal of ATP from the reaction mixture abolishes the observed unwinding activity. |
complete | |||||
| GO:0016787 |
hydrolase activity |
ECO:0000314 |
F |
Domains 2a and B shown in figure 3 once combined create architecture this is typical of monomeric helicase . |
complete | |||||
| GO:0016787 |
hydrolase activity |
ECO:0000314 |
F |
figure 2 shows both primary and secondary structure assignment of entire molecule. A1, A2, B1,B2 reflects domain locations that are consistent with monomeric helicases |
complete | |||||
|
NOT |
GO:0009379 |
Holliday junction helicase complex |
ECO:0000315 |
C |
Figure 5: In the presence of ATP and wild type UvsW branch migration of DHJS-2 Holliday junction occurs, whereas branch migration does not occur in the presence of K141R mutant of UvsW and even in the presence of UvsW when ATP is absent. |
complete | ||||
|
enables |
GO:0003677 |
DNA binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0005524 |
ATP binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016787 |
hydrolase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016787 |
hydrolase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0004386 |
helicase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0000166 |
nucleotide binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0005524 |
ATP binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ Carles-Kinch, K et al. (1997) Bacteriophage T4 UvsW protein is a helicase involved in recombination, repair and the regulation of DNA replication origins. EMBO J. 16 4142-51 PubMed GONUTS page
- ↑ 2.0 2.1 Kerr, ID et al. (2007) Crystallographic and NMR analyses of UvsW and UvsW.1 from bacteriophage T4. J. Biol. Chem. 282 34392-400 PubMed GONUTS page
- ↑ Webb, MR et al. (2007) The phage T4 protein UvsW drives Holliday junction branch migration. J. Biol. Chem. 282 34401-11 PubMed GONUTS page
