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BPT4:RIR1
Contents
| Species (Taxon ID) | Enterobacteria phage T4 (Bacteriophage T4). (10665) | |
| Gene Name(s) | NRDA | |
| Protein Name(s) | Ribonucleoside-diphosphate reductase subunit alpha
Protein B1 Ribonucleotide reductase | |
| External Links | ||
| UniProt | P32282 | |
| EMBL | J03968 AF158101 M22627 | |
| RefSeq | NP_049845.1 | |
| ProteinModelPortal | P32282 | |
| GeneID | 1258795 | |
| UniPathway | UPA00326 | |
| Proteomes | UP000009087 | |
| GO | GO:0005524 GO:0004748 GO:0006260 | |
| InterPro | IPR005144 IPR013346 IPR000788 IPR013509 IPR008926 | |
| Pfam | PF03477 PF02867 PF00317 | |
| PRINTS | PR01183 | |
| SUPFAM | SSF48168 | |
| TIGRFAMs | TIGR02506 | |
| PROSITE | PS51161 PS00089 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0005971 |
ribonucleoside-diphosphate reductase complex |
ECO:0000314 |
C |
Figure 4 shows that NrdA and NrdB from bacteriophage T4 have no activity alone; both are necessary to form a complex capable of ribonucleoside-diphosphate reductase activity |
complete | |||||
| GO:0004748 |
ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor |
ECO:0000314 |
F |
Both nrdA and nrdB were cleaved out of T4 phage genome. They were then put into a plasmid vector (pnrdAB)and implanted into E. coli N4830. Expression was induced by increasing temperature. Ribonucleoside diphosphate reductase was observed to increase in the E. coli strains containing the pnrdAB vector. |
complete | |||||
| GO:0055114 |
oxidation-reduction process |
ECO:0000314 |
P |
Figure 2 shows allosteric substrate specificity site is occupied by the effector molecule dTTP. |
complete | |||||
|
enables |
GO:0004748 |
ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0004748 |
ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0005524 |
ATP binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006260 |
DNA replication |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0055114 |
oxidation-reduction process |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0004748 |
ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor |
ECO:0000501 |
evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0055114 |
oxidation-reduction process |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0008152 |
metabolic process |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016491 |
oxidoreductase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006260 |
DNA replication |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniProtKB-KW:KW-0235 |
P |
Seeded From UniProt |
complete | ||
|
enables |
GO:0000166 |
nucleotide binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003824 |
catalytic activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0005524 |
ATP binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ Berglund, O (1975) Ribonucleoside diphosphate reductase induced by bacteriophage T4. III. Isolation and characterization of proteins B1 and B2. J. Biol. Chem. 250 7450-5 PubMed GONUTS page
- ↑ 2.0 2.1 Tseng, MJ et al. (1992) Tandem cloning of bacteriophage T4 nrdA and nrdB genes and overproduction of ribonucleoside diphosphate reductase (alpha 2 beta 2) and a mutationally altered form (alpha 2 beta 2(93)). J. Bacteriol. 174 5740-4 PubMed GONUTS page
- ↑ Eriksson, M et al. (1997) Binding of allosteric effectors to ribonucleotide reductase protein R1: reduction of active-site cysteines promotes substrate binding. Structure 5 1077-92 PubMed GONUTS page
