BPT4:DNLI

Species (Taxon ID)	Enterobacteria phage T4 (Bacteriophage T4). (10665)
Gene Name(s)	30
Protein Name(s)	DNA ligase Polydeoxyribonucleotide synthase [ATP]
External Links
UniProt	P00970
EMBL	X00039 AF158101
PIR	A01201
RefSeq	NP_049813.1
ProteinModelPortal	P00970
BindingDB	P00970
ChEMBL	CHEMBL5733
GeneID	1258680
KEGG	vg:1258680
KO	K18951
OrthoDB	VOG09000097
PRO	PR:P00970
Proteomes	UP000009087
GO	GO:0005524 GO:0003910 GO:0046872 GO:0051103 GO:0006310 GO:0006260
InterPro	IPR012310 IPR016059 IPR012340
Pfam	PF01068
SUPFAM	SSF50249
PROSITE	PS00697 PS00333

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
	GO:0003909	DNA ligase activity	PMID:16302964	ECO:0000314			F		In figure 2, single stranded DNA was mixed with T4 DNA ligase and oligonucleotides. In 2A, it was amplified using PCR. The resulting products were run through a gel. Lanes 1 and 6 were used as controls, either missing the ligase or the oligonucleotides. Lanes 3-5 showed the results from the PCR. The smear corresponds with the DNA ligase binding sequences of DNA to the experimental ssDNA, making the DNA longer and so the bands would have a larger masses. Figure 2B shows this better, as RCA was used to amplify the ssDNA, which is better to use for circular DNA. Distinct bands were seen due to this better fitting technique. The larger masses of the circular DNA also supports the fact that the DNA ligase can bind DNA ends together.	complete CACAO 13130
enables	GO:0003909	DNA ligase activity	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR016059	F		Seeded From UniProt	complete
enables	GO:0003910	DNA ligase (ATP) activity	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR012310	F		Seeded From UniProt	complete
enables	GO:0005524	ATP binding	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR012310	F		Seeded From UniProt	complete
involved_in	GO:0006281	DNA repair	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR012310	P		Seeded From UniProt	complete
involved_in	GO:0006310	DNA recombination	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR012310	P		Seeded From UniProt	complete
involved_in	GO:0051103	DNA ligation involved in DNA repair	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR016059	P		Seeded From UniProt	complete
enables	GO:0003910	DNA ligase (ATP) activity	GO_REF:0000003	ECO:0000501	evidence used in automatic assertion	EC:6.5.1.1	F		Seeded From UniProt	complete
enables	GO:0046872	metal ion binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0479	F		Seeded From UniProt	complete
involved_in	GO:0006310	DNA recombination	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0233	P		Seeded From UniProt	complete
involved_in	GO:0006281	DNA repair	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0234	P		Seeded From UniProt	complete
involved_in	GO:0006974	cellular response to DNA damage stimulus	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0227	P		Seeded From UniProt	complete
enables	GO:0000166	nucleotide binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0547	F		Seeded From UniProt	complete
enables	GO:0005524	ATP binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0067	F		Seeded From UniProt	complete
enables	GO:0016874	ligase activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0436	F		Seeded From UniProt	complete
involved_in	GO:0006260	DNA replication	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0235	P		Seeded From UniProt	complete
edit table

References

See Help:References for how to manage references in GONUTS.