GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
BPT1:SPANU
Contents
| Species (Taxon ID) | Escherichia phage T1 (Bacteriophage T1). (1921008) | |
| Gene Name(s) | No Information Provided. | |
| Protein Name(s) | U-spanin
Gene product 11 (ECO:0000305) Unimolecular spanin (ECO:0000303 with PMID:30135120[1]) gp11 (ECO:0000303 with PMID:30135120[1]) | |
| External Links | ||
| UniProt | Q6XQ97 | |
| EMBL | AY216660 | |
| RefSeq | YP_003934.1 | |
| TCDB | 1.M.2.1.1 | |
| GeneID | 2773029 | |
| KEGG | vg:2773029 | |
| OrthoDB | 13958at10239 | |
| Proteomes | UP000001156 | |
| GO | GO:0020002 GO:0016021 GO:0019835 GO:0044659 | |
| PROSITE | PS51257 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0020002 |
host cell plasma membrane |
C |
Figure 3. "T4 T holin accumulates in the inner membrane [...]". |
Missing: evidence | ||||||
| GO:0034291 |
canonical holin activity |
|
F |
Figure 2A. and Figure 2B. show that the T4 protein "t" can complement the function of Lambda "S" holin gene. |
Missing: evidence | |||||
| GO:0044659 |
cytolysis by virus of host cell |
UniProtKB:P00726 UniProtKB:Q37935
|
P |
Figure 4. shows the function of T1 gp11 restore the function of lambda Rz and Rz1. |
Missing: evidence | |||||
| GO:0045203 |
integral component of cell outer membrane |
|
C |
The T1 gp11 was found to have a sequence that very likely predicts an outer membrane lipoprotein, just as Lambda Rz1 produces an outer membrane lipoprotein component of a spanin (p. 1098). In addition, many phages, including Lambda, have a lysis cassette, which means that the holin, endolysin, and spanin are all next to each other in the genome (p. 1100). In the case of T1, genes 12 and 13 are the endolysin and holin, respectively, providing further evidence that gp11 is an integral component of the cell outer membrane. (I've also requested a new GO term to use for gp11, since this paper indicates that the protein disrupts the outer membrane. The issue can be found here https://github.com/geneontology/go-ontology/issues/12424). |
Missing: evidence | |||||
| GO:0045918 |
negative regulation of cytolysis |
ECO:0007699 |
cell growth assay evidence used in manual assertion |
P |
Fig. 4D shows GTG-to-GTC mutations in downstream alternative starts Val 63 and Val 65 cause early onset of lysis. Isoform gp11a (Q6XQ97-2) V63M Isoform gp11a' (Q6XQ97-3) V65M Organism: Escherichia phage T1 (Bacteriophage T1) |
complete | ||||
| GO:0044659 |
cytolysis by virus of host cell |
ECO:0007699 |
cell growth assay evidence used in manual assertion |
P |
Fig 3B. shows PRE plasmids containing the gp11 U-spanin complement MC4100 λRzamRz1am lysogens to rescue the wt lysis phenotype. Organism: Escherichia phage T1 (Bacteriophage T1) |
complete | ||||
|
part_of |
GO:0020002 |
host cell plasma membrane |
GO_REF:0000037 |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniProtKB-KW:KW-1030 |
C |
Seeded From UniProt |
complete | |
|
part_of |
GO:0016021 |
integral component of membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0019835 |
cytolysis |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
| GO:0045203 |
integral component of cell outer membrane |
ECO:0000317 |
genomic context evidence used in manual assertion |
C |
The T1 gp11 was found to have a sequence that very likely predicts an outer membrane lipoprotein, just as Lambda Rz1 produces an outer membrane lipoprotein component of a spanin (p. 1098). In addition, many phages, including Lambda, have a lysis cassette, which means that the holin, endolysin, and spanin are all next to each other in the genome (p. 1100). In the case of T1, genes 12 and 13 are the endolysin and holin, respectively, providing further evidence that gp11 is an integral component of the cell outer membrane. (I've also requested a new GO term to use for gp11, since this paper indicates that the protein disrupts the outer membrane. The issue can be found here https://github.com/geneontology/go-ontology/issues/12424). |
complete | ||||
|
part_of |
GO:0033644 |
host cell membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0090680 |
disruption by virus of host outer membrane |
ECO:0000317 |
genomic context evidence used in manual assertion |
P |
Missing: with/from | |||||
|
part_of |
GO:0016020 |
membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 1.2 1.3 Kongari, R et al. (2018) Localization and Regulation of the T1 Unimolecular Spanin. J. Virol. 92 PubMed GONUTS page
- ↑ Ramanculov, E & Young, R (2001) An ancient player unmasked: T4 rI encodes a t-specific antiholin. Mol. Microbiol. 41 575-83 PubMed GONUTS page
- ↑ Ramanculov, E & Young, R (2001) Functional analysis of the phage T4 holin in a lambda context. Mol. Genet. Genomics 265 345-53 PubMed GONUTS page
- ↑ 4.0 4.1 4.2 4.3 Summer, EJ et al. (2007) Rz/Rz1 lysis gene equivalents in phages of Gram-negative hosts. J. Mol. Biol. 373 1098-112 PubMed GONUTS page
