BPP22:VG10

Species (Taxon ID)	Enterobacteria phage P22 (Bacteriophage P22). (10754)
Gene Name(s)	10
Protein Name(s)	Packaged DNA stabilization protein gp10
External Links
UniProt	P26749
EMBL	L14810 AF217253 M59749 BK000583
PIR	S34955
RefSeq	NP_059633.1
GeneID	1262840
KEGG	vg:1262840
Proteomes	UP000001795 UP000007960
InterPro	IPR021098
Pfam	PF11134

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
	GO:0019073	viral DNA genome packaging	PMID:6363718	ECO:0000315			P		Fro. 4. Dependence of in vitro a.~.~em!~ly on the concentration of the 4", lO ÷ and 26* activities. (a) Com|)lementation of 10- extracts (O), 26- extracts (11) and 4- extracts (~lk) with protein donor extract. The cap,aid extracts were diluted 25-fold, to a final infected cell cont'entration of about 2 x 10 ° c~lls/ml. Samples were mixed with serial dilutions of the 5- prc)tein donor. The maximum slo|~s determined from these curve,a were: 4+ activity, 5 to 6; 26 + activity. I to 2; 10 + activity, 4 to 5. The background of the undiluted extracts were protein donor 10a; 4- extract < 10s; 10- extracts, 5 x 10~; 26- extract, 2 x l0 a. (b) Variation of the ratio of cap`aid,a to protein; complemcntation of 4- ~'apsids with cap,aid donor cell concentration: (O) 5x lO 9 cell,a/ml; (A) 2 x l0 s cell,a/ml; (m) 4 x i0 v (~lls/ml. The protein donor extract was from the same batch a.s used in (a). ependenc~ of phage formation in vitro on the concentration of the capsid donor extract. Infected ¢~zl[ extracts containing incomplete capsids were freshly thawed and lysed as described in Materials and Methods. D~uted .samples were mixed with concentrated protein donor extract {5-) and incubated for I h at 23°C, at which time the reactions were stopped by further dilution and titered for viable phage. The background of the protein donor extract was 102 phage/mi. The background of the • at mo.~l, Conc-entrated calmid donor extracts were (0) 3.3× 10 (4- extract), (t) 2:5 × l0 T (10- extract) and (at) 2 x 103 (26-extract).	complete CACAO 12033
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References

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