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BPMD2:VG11
Contents
| Species (Taxon ID) | Mycobacterium phage D29 (Mycobacteriophage D29). (28369) | |
| Gene Name(s) | 11 | |
| Protein Name(s) | Gene 11 protein
Gp11 | |
| External Links | ||
| UniProt | O64204 | |
| EMBL | AF022214 | |
| PIR | H72800 | |
| RefSeq | NP_046826.1 | |
| TCDB | 1.E.36.1.7 | |
| GeneID | 1261605 | |
| KEGG | vg:1261605 | |
| Proteomes | UP000002131 | |
| InterPro | IPR032121 | |
| Pfam | PF16081 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
NOT |
GO:0051715 |
cytolysis in other organism |
IDA: Inferred from Direct Assay |
P |
Figure 2: - (A) The growth curve was recorded after initiating HolFL expression in E. coli BL21(DE3) (in the case of pET and pTrc vectors) and TOP10 cells (in the case of pBAD vector), at time 0, by adding IPTG (+IPTG) and arabinose (+ara), respectively. * Measurement of E. coli when exposed to full-length Gp11. Host bacteria count decreased when exposed to gp11. - (C) The growth curve of M. smegmatis expressing full-length Gp11 is recorded after induction with 0.2% acetamide at time 0. * Host (M. smeg) bacterial count decreased with addition of inducer acetamide. - (E) Growth kinetics was recorded 1 min for 60 min after induction at time 0, in a 96-well plate, for E. coli carrying specified vectors. * Gp11 Plasmid standalone and gp11 plasmid combination (with gp10) cause lysis in host bacteria of both M.smeg and E. coli Quotes found in the results section give overview of cytolysis function of gp11 in phage D29: - "we find that the expression of D29 gp11 is sufficient to kill [via gp11 mediated lysis] E. coli or M. smeg." - "Gp10 and Gp11 in E. coli results in enhanced killing of bacterial cells." |
complete | ||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ Kamilla, S & Jain, V (2016) Mycobacteriophage D29 holin C-terminal region functionally assists in holin aggregation and bacterial cell death. FEBS J. 283 173-90 PubMed GONUTS page
