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AMYMU:D8HZ95

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Species (Taxon ID) Amycolatopsis mediterranei (strain U-32). (749927)
Gene Name(s) rifN (ECO:0000313 with EMBL:ADJ42450.1)
Protein Name(s) Kanosamine kinase (ECO:0000313 with EMBL:ADJ42450.1)
External Links
UniProt D8HZ95
EMBL CP002000
RefSeq WP_013222560.1
YP_003762852.1
ProteinModelPortal D8HZ95
EnsemblBacteria ADJ42450
GeneID 9434848
KEGG amd:AMED_0630
PATRIC 42148187
HOGENOM HOG000150087
KO K16018
OMA DRTFRWP
BioCyc AMED749927:GCZN-639-MONOMER
Proteomes UP000000328
GO GO:0016301
InterPro IPR000600
Pfam PF00480

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
GO:0016301

kinase activity

PMID:12207505[1]

ECO:0000314

F

The group studying RifN first purified the RifN protein and used a coupling assay to measure phosporylation with NADH consumption. The group used many different carbohydrates (kanosamine, glucose, fructose, mannose, galactose, glucosamine, 3-amino-3-deoxy-D-fructose) and found that there was only a change in absorance found with kanosamine as seen in figure 1. This specifies that RifN is only specific to kinase activity with kanosamine to form kanosamine-6-phosphate. The product formation was determined by H1 NMR and C13 NMR to determine the structure of the product.

complete

involved_in

GO:0016310

phosphorylation

GO_REF:0000038

ECO:0000323

imported automatically asserted information used in automatic assertion

UniProtKB-KW:KW-0418

P

Seeded From UniProt

complete

enables

GO:0016301

kinase activity

GO_REF:0000038

ECO:0000323

imported automatically asserted information used in automatic assertion

UniProtKB-KW:KW-0418

F

Seeded From UniProt

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. Arakawa, K et al. (2002) Characterization of the early stage aminoshikimate pathway in the formation of 3-amino-5-hydroxybenzoic acid: the RifN protein specifically converts kanosamine into kanosamine 6-phosphate. J. Am. Chem. Soc. 124 10644-5 PubMed GONUTS page