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9CAUD:R9VY83

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Species (Taxon ID) Paenibacillus phage phiIBB_Pl23. (1337877)
Gene Name(s) No Information Provided.
Protein Name(s) N-acetylmuramoyl-L-alanine amidase (ECO:0000313 with EMBL:AGN89335.1)
External Links
UniProt R9VY83
EMBL KF010834
RefSeq YP_008320357.1
GeneID 16385281
KEGG vg:16385281
Proteomes UP000014424
GO GO:0008745
GO:0009253
CDD cd06583
Gene3D 3.40.80.10
InterPro IPR002502
Pfam PF01510
SMART SM00644
SUPFAM SSF55846

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
GO:0008745

N-acetylmuramoyl-L-alanine amidase activity

PMID:26167894[1]

ECO:0000314

F

The authors claim that 'BlastP, Pfam and HHpred identified an N-acetylmuramoyl-L-alanine amidase belonging to the Amidase_2 family (Pfam 01510) at the N-terminus encompassing two third of the protein. CDD database identified 5 putative amidase catalytic residues: two histidines (position 29 and 129), a phenylalanine (position 53), a lysine (position 135) and a cysteine (position 137)' (First paragraph of the results section).

Table 2 however confirmed that PlyPl23 has lytic activity and also shows the lytic spectrum of PlyPl23 when assessed against a panel of 20 P. larvae isolates and 4 additional strains belonging

to other related bacterial species (Bacillus cereus, Lactobacillus paracasei, 

Lactobacillus pentosus, Bacillus subtilis), and compared with that of the respective phage phiIBB_Pl23. Results showed that while the phage phiIBB_Pl23 was able to lyse 16 of the 20 strains (80%), its endolysin (PlyPl23) was active against all the tested Paenibacillus larvae strains. The Bacillus and Lactobacillus strains were not lysed by the phage or by the enzyme

complete
CACAO 12773

enables

GO:0008745

N-acetylmuramoyl-L-alanine amidase activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR002502
InterPro:IPR036505

F

Seeded From UniProt

complete

involved_in

GO:0009253

peptidoglycan catabolic process

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR002502
InterPro:IPR036505

P

Seeded From UniProt

complete

enables

GO:0008745

N-acetylmuramoyl-L-alanine amidase activity

GO_REF:0000003

ECO:0000501

evidence used in automatic assertion

EC:3.5.1.28

F

Seeded From UniProt

complete

enables

GO:0016787

hydrolase activity

GO_REF:0000038

ECO:0000323

imported automatically asserted information used in automatic assertion

UniProtKB-KW:KW-0378

F

Seeded From UniProt

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. Oliveira, A et al. (2015) The First Paenibacillus larvae Bacteriophage Endolysin (PlyPl23) with High Potential to Control American Foulbrood. PLoS ONE 10 e0132095 PubMed GONUTS page