9CAUD:M1EAF4

Species (Taxon ID)	Salmonella phage vB_SenMS16. (1087482)
Gene Name(s)	No Information Provided.
Protein Name(s)	Uncharacterized protein (ECO:0000313 with EMBL:AEO97040.1)
External Links
UniProt	M1EAF4
EMBL	HQ331142
RefSeq	YP_007501094.1
GeneID	14675295
KEGG	vg:14675295
Proteomes	UP000011284
Gene3D	1.10.600.10
InterPro	IPR022558 IPR008949
Pfam	PF10849
ProDom	PD069849

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
	GO:0098024	virus tail, fiber	PMID:23289425	ECO:0000314			C		In silico analysis was used to identify gp38 as a component of the putative long tail fibre (LTF) (Figure 3). Sequence identity of S16 gp38 to the corresponding gene products in phages T4 and T2 is high (46%)(Figure 3). A phylogenetic comparison of gp38 of S16 to homologues of other T4-like phages also clusters the S16 variant closer to T2 than to T4 (Figure S2). SDS-PAGE of different S16 GFP-LTF protein synthesis variations in E.coli resulted in the production of functional GFP–LTF and indicates trimerization of S16 gp37 and attachment of gp38 to S16 gp37 trimers (Figure 4 and S1) The distal part of the S16 LTF likely has a putative structure closely related to what has been reported for phage T2 (Drexler et.al., 1986), where gp38 acts as the actual adhesin while bound to the C-terminal (distal) tip of gp37. The binding of purified S16 GFP–LTF fusion protein to wild-type and mutant Salmonella typhimurium cells (with mutated OmpC) were assessed. (Figure?5A and B) The wild-type revealed an even decoration of the bacterial surface by the fluorescent LTF (Figure?5A and B). Deletion of OmpC completely abolished decoration by S16 GFP–LTF (Fig.?5C and D). The defective phenotype could be restored by in-trans complementation using ompC (Table?3, Figure?5E and F).	complete CACAO 12625
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References

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