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9BACT:I6N3G4

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Species (Taxon ID) uncultured bacterium pDL136. (1071409)
Gene Name(s) estDL136 (ECO:0000313 with EMBL:AEO37498.1)
Protein Name(s) Esterase EstDL136 (ECO:0000313 with EMBL:AEO37498.1)
External Links
UniProt I6N3G4
EMBL JN242251
GO GO:0016787
Gene3D 3.40.50.1820
InterPro IPR029058
IPR013094
Pfam PF07859
SUPFAM SSF53474

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
GO:0016810

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

PMID:22752166[1]

ECO:0000314

F

Figure 1 shows degradation of chloramphenicol in the presence of cell extract containing DL136. Figure 2 shows the mass spec and NMR for both the hydrosylate and the nitrophenylserinol standard. These are identical, showing that DL136 hydrolyzed the amide bond in chloramphenicol.

complete
CACAO 10677

enables

GO:0016787

hydrolase activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR013094

F

Seeded From UniProt

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. Tao, W et al. (2012) Inactivation of chloramphenicol and florfenicol by a novel chloramphenicol hydrolase. Appl. Environ. Microbiol. 78 6295-301 PubMed GONUTS page