UMBC Phage Hunters Spring 2015
My Annotations
| Status | Page | Date/Time | GO Term (Aspect) | Reference | Evidence | Notes | Links |
|---|
| acceptable | BACSU:RIR1 | 2015-04-06 10:55:09 CDT | GO:0004748 ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor (F) | PMID:8969495 | ECO:0000315 mutant phenotype evidence used in manual assertion | This paper follows the studies in another article (PMID 4627024) which I don't have access to read past the beginning results and the abstract. This previous article used a temperature sensitive mutant ts-167 to determine the function of the nrdEF locus. That paper determined the function by analyzing the pool of dNTP present at the restrictive temperature. dCTP and dATP were not apparent at these restrictive temperatures.
The reference listed above (PMID 8969495) used phylogeny and sequencing to determine that Enterobacteria favor the use of nrdAB loci, whereas Bacilus subtils has evolved to use nrdEF. They also used clone pMP25.8 to rescue the temperature sensitive mutant, and later sequenced the nrdE mutant to determine there were 2 neutral mutations and 1 mutation that led to the lethality of the temperature sensitive mutant. They concluded that this mutation was the cause for the loss of function of the NrdE protein.
The mutant lethality due to the loss of dCTP and dATP synthesis provides evidenct that this gene is responsible for the essential enzyme responsible for reductase activity for the respective ribonucleoside-diphosphates.
| challenge |
| unacceptable | 9CAUD:S5YPJ2 | 2015-04-08 10:11:18 CDT | GO:0004748 ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor (F) | GO_REF:0000100 | ECO:0000266 sequence orthology evidence used in manual assertion | The BLASTp e-values for Troll gene 127 blasted against Bacillus strain 168 gave a value of 4e^-15. When reversed the BLAST from strain 168 to Troll, e-value was 2e^-14. The BLASTp results show orthology for nrdE of Bacillus strain 168.
| challenge |
acceptable:1
unacceptable:1
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flagged:0
Annotations challenged by Sjesse
| Status | Author,Group | Page | GO Term (Aspect) | Reference | Evidence | Links | Page history |
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0 annotations fixed by Sjesse
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