User:Shabali1

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	CORGL:Q8NP42	2016-04-10 02:07:23 CDT	GO:0004309 exopolyphosphatase activity (F)	PMID:19304823	ECO:0000315 mutant phenotype evidence used in manual assertion	The deletion of ppx1 and ppx2 in C. glutamicum resulted in the decrease of exopolyphosphatase activity. Figure 1 shows that there was an accumulation of cellular PolyP mutated C. glutamicum cells versus wild type. This shows that the exopolyphosphatase protein serves as a regulatory protein of cellular PolyP	challenge
updatedbyinstructor	CORGL:Q8NRR8	2016-04-11 04:28:29 CDT	GO:0004309 exopolyphosphatase activity (F)	PMID:19304823	ECO:0000314 direct assay evidence used in manual assertion	Different amounts of PolyPs were added in chains in the medium containing the PPX2His (exopolyphosphatase with a His tag) to see if an increase in the length of the PolyP chain increased the activity if the PPX2 activity. According to the results in Figure 3, the increase in the length (from short to medium) increased the percent of activity. Therefore, the enzyme activity depends on the amount of cellular PolyP available.	challenge

acceptable:1
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Shabali1

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
unacceptable	Omololu1, Team Blue B	9CAUD:A0A0K2D0J0	GO:0000166 - nucleotide binding (F)	PMID:17209017	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 3

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