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User:Kothaa

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Phage Hunters Spring 2017

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesBPBPP:Q775C72017-04-05 13:52:46 CDTGO:0019012 virion (C)PMID:24347545ECO:0000314 direct assay evidence used in manual assertion

Figure 1 shows the results of mass spectrometry from the major band of a gel that was derived from whole phage particles. From the thick black line under part B on the top of the mass spectrometry, it is evident that Bbp16 is the second most abundant protein. Mass spectrometry confirmed that the sequence obtained from the second most abundant band corresponded to capsid protein. This is a virion component because whole phage components were run as a gel.The band migrating at 15.0 kD is around the size they expected based on the sequence of the capsid.

challenge
unacceptableBPBPP:Q775C72017-04-30 21:30:00 CDTGO:0019028 viral capsid (C)other:ECO:0000250 sequence similarity evidence used in manual assertion

The sequence of the protein Bbp16 from the phage Bordetella phage BPP-1 was run in the BLASTp program to analyze its similarity to the protein sequences of other phages with major capsid protein sequences. The results indicated a close relationship between the major capsid protein sequences of Bordetella phage BPP-1 and Bordetella avium (strain 197N) based on the percent identity, the e-value, and the query coverage percentage. The percent identity of Bordetella avium to phage BPP-1 is 97.3% which indicates a very close similarity between the two sequences. The e-value is 0.00, and the query coverage is 100. The e-value, high query coverage, and high percent identity collectively indicate that there is a major capsid protein in Boredatella phage BPP-1.

challenge

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