Phage Hunters Spring 2017
My Annotations
| Status | Page | Date/Time | GO Term (Aspect) | Reference | Evidence | Notes | Links |
|---|
| unacceptable | BACAN:Q81YZ2 | 2017-04-17 10:24:28 CDT | GO:0016998 cell wall macromolecule catabolic process (P) | PMID:21816821 | ECO:0000314 direct assay evidence used in manual assertion | The effect of B-anthracis s-layer on lytic activity and whole cell binding by using two homologous amidases plyL and plyG are show in these figures. Figure 5A: shows the pulling down of whole cell wall binding assay using a tagged CBD of plyL and plyG infecting wild B. anthracis, B. cereus 4342 B. and subtilis. Also using it as a control as a architecture. Lower gels are Western blots using anti-His antibody, and the Upper gels show expression of Sap protein, with markers at 59 and 107 kDa.
| challenge |
| unacceptable | BACAN:Q81YZ2 | 2017-04-26 08:58:14 CDT | GO:0016998 cell wall macromolecule catabolic process (P) | PMID:21816821 | ECO:0000314 direct assay evidence used in manual assertion | the Effect of S-layer—Was compared the effects of PlyL and they were closely related PlyG with 96% identical in the catalytic domain , and 63% in the CBD.
Figure 5A: shows how the cell killing activity of the full length which is the top bar and the catalytic domain the second bar of plyL and plyG against B. anthracis Sterne, B. anthracis (sap) mutant deficient in S-layer synthesis,and B. subtilis. Each B strains consist of 0.4uM of lysin concentration. From the graph we can also conclude that PlyG(CAT) killed Sterne more than twice as fast as PlyG(FULL). The full length graph show that with out S-layer, PlyL and plyG can still be active. The blue bar is plyL (full) and red bar is plyL(CAT). the light blue bar is plyG (full) and green bar is plyG(CAT). PlyL(CAT) had a higher lytic activity than PlyL(FULL), and PlyG(CAT) killed Sterne more than twice as fast as
PlyG (FULL)
| challenge |
acceptable:0
unacceptable:2
requires_changes:0
flagged:0
Annotations challenged by Gbadamosiz
| Status | Author,Group | Page | GO Term (Aspect) | Reference | Evidence | Links | Page history |
|---|
0 annotations fixed by Gbadamosiz
What do the icons mean in the status column?
